Planta

, Volume 144, Issue 5, pp 427–435 | Cite as

Analysis of gametophytic development in the moss, Physcomitrella patens, using auxin and cytokinin resistant mutants

  • N. W. Ashton
  • N. H. Grimsley
  • D. J. Cove
Article

Abstract

Mutants altered in their response to auxins and cytokinins have been isolated in the moss Physcomitrella patens either by screening clones from mutagenized spores for growth on high concentrations of cytokinin or auxin, in which case mutants showing altered sensitivities can be recognized 3–4 weeks later, or by non-selective isolation of morphologically abnormal mutants, some of which are found to have altered sensitivities. Most of the mutants obtained selectively are also morphologically abnormal. The mutants are heterogeneous in their responses to auxin and cytokinin, and the behaviour of some is consistent with their being unable to make auxin, while that of others may be due to their being unable to synthesize cytokinin. Physiological analysis of the mutants has shown that both endogenous auxin and cytokinin are likely to play important and interdependent roles in several steps of gametophytic development. Although their morphological abnormalities lead to sterility, genetic analysis of some of the mutants has been possible by polyethyleneglycol induced protoplast fusion.

Key words

Auxin Cytokinin Mutants Physcomitrella Protoplast fusion 

Abbreviations

NTG

N-methyl-N′-nitro-N-nitrosoguanidine

NAA

1-naphthalene acetic acid

2,4D

2,4-dichlorophenoxyacetic acid

BAP

6-benzylaminopurine

IAP

6-(Δ2isopentenyl) aminopurine

NAR

NAA resistant mutants

BAR

BAP resistant mutants

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • N. W. Ashton
    • 1
  • N. H. Grimsley
    • 1
  • D. J. Cove
    • 1
  1. 1.Department of GeneticsUniversity of CambridgeCambridgeU.K.

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