, Volume 61, Issue 1, pp 115–121 | Cite as

Inorganic carbon assimilation in the Isoetids, Isoetes lacustris L. and Lobelia dortmanna L.

  • K. Richardson
  • H. Griffiths
  • M. L. Reed
  • J. A. Raven
  • N. M. Griffiths
Original Papers


The inorganic carbon fixation patterns of Isoetes lacustris and Lobelia dortmanna from an oligotrophic Scottish loch have been examined by following titratable acidity changes in plant sap and light/dark 14CO2 incorporation by roots and shoots. The diurnal pattern of titratable acidity changes in I. lacustris suggests crassulacean acid metabolism (CAM) while the lack of any change in titratable acidity in L. dortmanna suggests C3 metabolism. Of the carbon fixed by L. dortmanna, 99.9% was taken up through the roots and fixation occurred primarily during the day. In Isoetes, CO2 was taken up by both roots and shoots and during both day and night. Regardless of the site of CO2 uptake, fixation occurred only in the shoots of both plants. Analysis of carbon isotope ratios of plant organic material was used to further investigate the photosynthetic mechanisms of these Isoetids. Considering the absence of a nighttime peak in titratable acidity in L. dortmanna, the Δ13C (Δ=δ13C plant-δ13C source) value of the shoots of L. dortmanna (-14.2‰) is indicative of C3 photosynthesis limited by the rate of CO2 diffusion. The less negative Δ of I. lacustris (-6.0‰) is consistent with both dark acidification of CAM and CO2 limited C3 photosynthesis. This is in contrast to the terrestrial Isoetes durieui which is shown to have a Δ value which is similar to a terrestrial C3 plant. The carbon fixation patterns of these Isoetids suggest that the CO2 concentration in the loch may be growth limiting, and that root uptake and/or dark acidification are means of optimising CO2 supply. However, in view of the relatively high levels of CO2 in sediment and bulk water, it is suggested that low levels of nutrients may also limit growth in these plants.


Carbon Isotope Inorganic Carbon Titratable Acidity Crassulacean Acid Metabolism Carbon Isotope Ratio 
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  1. American Public Health Association (1976) Standard methods for the examination of water and wastewater, including bottom sediments and sludge, 14th edn. American Public Health Association New YorkGoogle Scholar
  2. Beer S, Wetzel RG (1981) Photosynthetic carbon metabolism in the submerged aquatic angiosperm Scirpus subterminalis. Plant Sci Lett 21:199–207Google Scholar
  3. Benzing DH, Pridgeon AM (1983) Foliar trichomes of Pleurothallidinae (Orchidaceae): functional significance. Am J Bot 70:173–181Google Scholar
  4. Black CC, Carnal NW, Kenyon WH (1982) Compartmentation and the regulation of CAM. In: Ting IP, Gibbs M (eds) Crassulacean Acid Metabolism. American Society of Plant Physiologists, Bethesda, pp 51–68Google Scholar
  5. Brown RH (1978) A difference in N use efficiency in C3 and C4 plants and its implications in adaptation and evolution. Crop Sci 18:93–98Google Scholar
  6. Clapham AR, Tutin TG, Warburg GF (1981) Excursion flora of the British Isles. Cambridge University PressGoogle Scholar
  7. Du Rietz EG (1921) Zur methodologischen Grundlage einer modernen Pflanzensoziologie. Thesis, UppsalaGoogle Scholar
  8. Du Rietz EG (1930) Vegetationforschung auf soziationanalytischer Grundlage. Abderhalden Handb Biol Arbeitsmeth 11 (5):293–480Google Scholar
  9. Farquhar GD (1980) Carbon isotope discrimination by plannts: effects of carbon dioxide concentration and temperature via the ratio of intercellular and atmospheric CO2 concentrations. In: Pearman GI (ed) Carbon dioxide and climate: Australian research. Australian Academy of Science, Canberra, pp 105–110Google Scholar
  10. Farquhar GD, O'Leary MH, Berry JA (1982) On the relationship between carbon isotope discrimination and intracellular carbon dioxide concentration in leaves. Aust J Plant Physiol 9:121–137Google Scholar
  11. Gessner F (1959) Hydrobotanik II, Stoffhaushalt. VEB Deutscher Verlag der Wissenschaften, BerlinGoogle Scholar
  12. Grime JP (1979) Plant strategies and vegetation processes. John Wiley and Sons, London New YorkGoogle Scholar
  13. Hartog C den, Segal S (1964) A new classification of the water plant communities. Acta Bot Neerl 13:367–393Google Scholar
  14. Haslam S, Sinker C, Wolseley P (1975) British water plants. Field Stud 4:242–351Google Scholar
  15. Holaday AS, Bowes G (1980) C4 acid metabolism and dark CO2 fixation in a submerged aquatic macrophyte (Hydrilla verticillata). Plant Physiol 65:331–335Google Scholar
  16. Holtum JAM, O'Leary MH, Osmond CB (1982) Carbon isotope fractionation during dark CO2 fixation in CAM plants. In: Ting IP, Gibbs M (eds) Crassulacean Acid Metabolism. American Society of Plant Physiologists. Bethesda, pp 299–300Google Scholar
  17. Hutchinson GE (1975) A treatise on limnology, Vol. III Limnological Botany. John Wiley and Sons, New YorkGoogle Scholar
  18. Keeley JE (1981a) Isoetes howellii: a submerged aquatic CAM plant. Am J Bot 68:420–424Google Scholar
  19. Keeley JE, (1981b) Diurnal acid metabolism in vernal pool Isoetes (Isoetaceae). Madroño 28:167–171Google Scholar
  20. Keeley JE (1982a) Distribution of diurnal acid metabolism in the genus Isoetes. Am J Bot 69:254–257Google Scholar
  21. Keeley JE (1982b) Crassulacean acid metabolism in submerged aquatic plants. In: Ting IP, Gibbs M (eds) Crassulacean Acid Metabolism. American Society of Plant Physiologists, Bethesda, pp 303–304Google Scholar
  22. Keeley JE (1983) Crassulacean acid metabolism in the seasonally submerged aquatic Isoetes howellii. Oecologia (Berlin) 58:57–62Google Scholar
  23. Keeley JE, Morton B, Babcock B, Castello P, Fish B, Jerauld E, Johnson B, Landre L, Lum M, Miller C, Parker A, van Steenwyck G (1981) Dark CO2 fixation in the submerged aquatic Isoetes storkii. Oecologia (Berlin) 48:332–333Google Scholar
  24. Keeley JE, Walker JM, Matthews RP (1983) Crassulacean acid Metabolism in Isoetes bolanderi in high elevation oligotrophic lakes. Oecologia (Berlin) 58:63–69Google Scholar
  25. Krom MD, Berner RA, (1980a) The diffusion coefficient of sulfate, ammomium and phosphate ions in anoxic marine sediments. Limnol Oceanogr 25:327–337Google Scholar
  26. Krom MD, Berner RA (1980b) Adsorption of phosphate in anoxic marine sediments. Limnol Oeceanogr 25:797–806Google Scholar
  27. Moeller RE (1978) Seasonal changes in biomass, tissue chemistry and net production of the evergreen hydrophyte, Lobelia dortmanna. Can J Bot 56:1425–1433Google Scholar
  28. Mook WG, Bommerson JC, Staverman WH (1974) Carbon isotope fractionation between dissolved bicarbonate and gaseous carbon dioxide. Earth Planet Sci Lett 22:169–176Google Scholar
  29. Nye DH, Tinker PB (1977) Solute movement in the soil-root system. Blackwell, OxfordGoogle Scholar
  30. O'Leary MH (1981) Carbon isotope fractionation in plants. Phytochemistry 20:553–568Google Scholar
  31. O'Leary MH, Osmond CB (1980) Diffusional contribution to isotope fractionation during dark CO2 fixation in CAM plants. Plant Physiol 66:931–934Google Scholar
  32. Osmond CB, Valaane N, Haslam SM, Uotila P, Roksandic Z (1981) Comparisons of δ13C values in leaves of aquatic macrophytes from different habitats in Britain and Finland; some implications for photosynthetic processes in aquatic plants. Oecologia (Berlin) 50:117–124Google Scholar
  33. Öztürk M, Rehder H, Ziegler H (1981) Biomass production of C3 plant and C4 plant species in pure and mixed culture with different water supply. Oecologia (Berlin) 50:73–81Google Scholar
  34. Raven JA (1970) Exogenous inorganic carbon sources in plant photosynthesis. Biol Rev 45:167–221Google Scholar
  35. Raven JA (1976) Transport in algal cells. In: Lüttge U, Pitman MG (eds) Encyclopedia of Plant Physiology (new series), Vol. 11A. Springer Verlag, Berlin Heidelberg New York, pp 129–188Google Scholar
  36. Raven JA (1981) Nutritional strategies of submerged benthic plants: the acquisition of C, N and P by rhizophytes and haptophytes. New Phytol 88:1–30Google Scholar
  37. Raven JA, Beardall J, Griffiths H (1982) Inorganic C-sources for Lemanea, Cladophora and Ranunculus in a fast flowing stream: measurements of gas exchange and of carbon isotope ratio and of their ecological implications. Oecologia (Berlin) 53:68–78Google Scholar
  38. Sand-Jensen K (1978) Metabolic adaptation and vertical zonation of Littorella uniflora (L) Aschers and Isoetes lacustris L. Aquat Bot 4:1–10Google Scholar
  39. Sand-Jensen K, Prahl C (1982) Oxygen exchange with the lacunae and across leaves and roots of the submerged macrophyte, Lobelia dortmanna L. New Phytol 91:103–120Google Scholar
  40. Sand-Jensen K, Prahl C, Stockholm H (1982) Oxygen release from roots of submerged aquatic macropytes. Oikos 38:349–354Google Scholar
  41. Sand-Jensen K, Søndergaard M (1978) Growth and production of Isoetids in oligotrophic Lake Kalgaard, Denmark. Verh Internat Verein Limnol 20:659–666Google Scholar
  42. Smith FA, Walker NA (1980) Photosynthesis by aquatic plants: effects of unstirred layers in relation to assimilation of CO2 and HCO3 and to carbon isotope discrimination. New Phytol 86:245–259Google Scholar
  43. Smith SE (1980) Mycorrhizas of autotrophic higher plants. Biol Rev 55:475–510Google Scholar
  44. Søndergaard M, Laegaard S (1977) Vesicular-arbuscular mycorrhiza in some aquatic vascular plants. Nature 268:232–233Google Scholar
  45. Søndergaard M, Sand-Jensen K (1979) Carbon uptake by leaves and roots of Littorella uniflora (L.) Aschers Aquat Bot 6:1–12Google Scholar
  46. Tutin TG, Heywood VH, Burges NA, Valentine DH, Walters SM, Webb DA (1964) Flora Europaea, Vol. 1. Cambridge University Press, pp 5–6Google Scholar
  47. Vogel GC (1980) Fractionation of the carbon isotopes during photosynthesis. Springer-Verlag, Berlin Heidelberg New YorkGoogle Scholar
  48. Westlake DF (1965) Some basic data for investigations of the productivity of aquatic macrophytes. Mem 1st Ital Idrobiol (Suppl) 18:229–248Google Scholar
  49. Winter K, Wallace BJ, Stocker GC, Roksandic Z (1983) Crassulacean acid metabolism in Australian vascular epiphytes and some related species. Oecologia (Berlin) 57:129–141Google Scholar
  50. Wium-Andersen S (1971) Photosynthetic uptake of free CO2 by the roots of Lobelia dortmanna. Physiol Plant 25:245–248Google Scholar
  51. Wium-Andersen S (Andersen JM (1972) The influence of vegetation on the redox profile of the sediment of Grane Langsø, a Danish Lobelia lake. Limnol Oceanogr 17:943–947Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • K. Richardson
    • 1
  • H. Griffiths
    • 1
  • M. L. Reed
    • 1
  • J. A. Raven
    • 1
  • N. M. Griffiths
    • 1
  1. 1.Department of Biological SciencesUniversity of DundeeDundeeScotland

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