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Responses of wild plants to nitrate availability

Relationships between growth rate and nitrate uptake parameters, a case study with two Bromus species, and a survey

Summary

Two annual species of Bromus, an invader (B. hordeaceus, ex B. mollis) and a non-invader (B. intermedius), were grown for 28 days in growth chambers, at 5 and 100 μM NO 3 - in flowing nutrient solution. No differences between the two species were observed at either NO 3 - level, in terms of relative growth rate (RGR) or its components, dry matter partitioning, specific NO 3 - absorption rate, nitrogen concentration, and other characteristics of NO 3 - uptake and photosynthesis. The effects of decreasing NO 3 - concentration in the solution were mainly to decrease the NO 3 - concentration in the plants through decreased absorption rate, and to decrease the leaf area ratio through increased specific leaf mass and decreased leaf mass ratio. Organic nitrogen concentration varied little between the two treatments, which may be the reason why photosynthetic rates were not altered. Consequently, RGR was only slightly decreased in the 5-μM treatment compared to the 100-μM treatment. This is in contrast with other species, where growth is reduced at much higher NO 3 - concentrations. These discrepancies may be related to differences in RGR, since a log-linear relationship was found between RGR and the NO 3 - concentration at which growth is first reduced. In addition, a strong linear relationship was found between the RGR of these species and their maximum absorption rate for nitrate, suggesting that the growth of species with low maximum RGR may be partly regulated by nutrient uptake.

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References

  1. Behaeghe TJ (1974) Experiments on the seasonal variations of grass growth. In: Iglovikov VG, Movsisyants AP (eds) Proceedings of the XIIth International Grassland Congress (vol 1, part 1), Moscow (11–20 June 1974), pp 268–281

  2. Bhat KKS, Nye PH, Brereton AJ (1979) The possibility of predicting solute uptake and plant growth response from independently measured soil and plant characteristics. VI. The growth and uptake of rape in solutions of constant nitrate concentration. Plant Soil 53:137–167

  3. Bloom AJ (1985) Wild and cultivated barleys show similar affinities for mineral nitrogen. Oecologia 65:555–557

  4. Bloom AJ, Chapin FS III (1981) Differences in steady-state net ammonium and nitrate influx by cold- and warm-adapted barley varieties. Plant Physiol 68:1064–1067

  5. Bradshaw AD, Chadwick MJ, Jowett D, Snaydon RW (1964) Experimental investigations into the mineral nutrition of several grass species. IV. Nitrogen level. J Ecol 52:665–677

  6. Brouwer R (1963) Some aspects of the equilibrium between overground and underground plants parts. Jaarb IBS:31–677

  7. Brouwer R (1966) Root growth of grasses and cereals. In: Milthorpe FL, Ivins JD (eds) The growth of cereals and grasses. Butterworths, London, pp 153–165

  8. Cataldo DA, Haroon M, Schrader LE, Youngs VL (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Comm Soil Sci Plant Anal 6:71–80

  9. Chapin FS III (1980) The mineral nutrition of wild plants. Ann Rev Ecol Syst 11:233–260

  10. Chapin FS III, Van Cleve K, Tryon PR (1986) Relationship of ion absorption to growth rate in taiga trees. Oecologia 69:238–242

  11. Chapin FS III, Walter CHS, Clarkson DT (1988) Growth response of barley and tomato to nitrogen stress and its control by abscisic acid, water relations and photosynthesis. Planta 173:352–366

  12. Clarkson DT (1986) Regulation of the absorption and release of nitrate by plant cells: a review of current ideas and methodology. In: Lambers H, Neeteson JJ, Stulen I (eds) Fundamental, ecological and agriculutral aspects of nitrogen metabolism in higher plants. Martinus Nijhoff Publishers, Dordrecht, pp 3–27

  13. Clarkson DT, Hopper MJ, Jones LHP (1986) The effect of root temperature on the uptake of nitrogen and the relative size of the root system in Lolium perenne. I. Solutions containing both NH 4 + and NO 3 - . Plant Cell Env 9:535–545

  14. Clement CR, Hopper MJ, Jones LHP (1978) The uptake of nitrate by Lolium perenne from flowing nutrient solution. I. Effect of NO 4 - concentration. J Exp Bot 29:453–464

  15. Cox WJ, Reisenauer HM (1973) Growth and ion uptake by wheat supplied nitrogen as nitrate, or ammonium, or both. Plant Soil 38:363–380

  16. Davidson RL (1969) Effect of root/leaf temperature differentials on root/shoot ratios in some pasture grasses and clover. Ann Bot 33:561–569

  17. Doddema H (1978) Uptake of nitrate by chlorate-resistant mutants of Arabidopsis thaliana (L.) Heynh. PhD University of Groningen, Groningen, The Netherlands

  18. Dunn OJ, Clark VA (1974) Applied statistics: analysis of variance and regression. John Wiley and Sons, Chichester

  19. Field C, Mooney HA (1986) The photosynthesis-nitrogen relationship in wild plants. In: Givnish TJ (ed) On the economy of plant form and function. Cambridge University Press, Cambridge, pp 25–55

  20. Freijsen AHJ, Otten H (1984) The effect of nitrate concentration in a flowing solution system on growth and nitrate uptake of two Plantago species. Plant Soil 77:159–169

  21. Grime JP (1977) Evidence for the existence of three primary strategies in plants and its relevance to ecological and evolutionary theory. Am Nat 111:1169–1193

  22. Hirose T, Kitajima K (1986) Nitrogen uptake and plant growth. I. Effect of nitrogen removal on growth of Polygonum cuspidatum. Ann Bot 58:479–486

  23. Huffaker RC, Rains DW (1978) Factors influencing nitrate acquisition by plants; assimilation and fate of reduced nitrogen. In: Nielsen DR, MacDonald JG (eds) Nitrogen in the environment (vol. 2): Soil-plant-nitrogen relationships. Academic Press, New York, pp 1–43

  24. Hunt R (1982) Plant growth curves. The functional approach to plant growth analysis. Edward Arnold, London, p 248

  25. Hunt R, Parsons IT (1974) A computer program for deriving growth-functions in plant growth-analysis. J Appl Ecol 11:297–307

  26. Hunt R, Parsons IT (1981) Plant growth analysis: users' instructions for the stepwise and spline programs. Unit of Comparative Ecology (NERC), University of Sheffield, Sheffield

  27. Ingestad T (1981) Nutrition and growth of birch and grey alder seedlings in low conductivity solutions and at varied relative rates of nutrient addition. Physiol Plant 52:454–466

  28. Isaac RA, Johnson WC (1976) Determination of total nitrogen in plant tissue using a block digester. J Assoc Anal Org Chem 59:98–100

  29. Jackson LE, Schimel JP, Firestone MK (1989) Short-term partitioning of ammonium and nitrate between plants and microbes in an annual grassland. Soil Biol Biochem (in press)

  30. Klepper B, Rickman RW, Peterson CM (1982) Quantitative characterization of vegetative development in small cereal grain. Agron J 74:789–792

  31. Koch GW, Winner WE, Nardone A, Mooney HA (1987) A system for controlling the root and shoot environment for plant growth studies. Envir Exp Bot 27:365–377

  32. Lancaster DL (1976) Ecological significance of nitrate uptake and reduction by three annual grasses. PhD, University of California, Davis

  33. Lee RB, Rudge KA (1986) Effects of nitrogen deficiency on the absorption of nitrate and ammonium by barley plants. Ann Bot 57:471–486

  34. Lycklama JC (1963) The absorption of ammonium and nitrate by perennial ryegrass. Acta Bot Neerl 12:361–423

  35. Marcus-Wyner L (1983) Influence of ambient acidity on the absorption of NO 3 - and NH 4 + by tomato plants. J Plant Nutr 6:657–666

  36. Mattsson M, Lundborg T, Larsson C-M (1988) Nitrate utilization in barley: relations to nitrate supply and light-dark cycles. Physiol Plant 73:380–386

  37. McCree KJ, Troughton JH (1966) Prediction of growth rate at different light levels from measured photosynthesis and respirations rates. Plant Physiol 41:559–566

  38. Rao KP, Rains DW (1976) Nitrate absorption by barley. I. Kinetics and energetics. Plant Physiol 57:55–58

  39. Rodgers CO, Barneix AJ (1988) Cultivar differences in the rate of nitrate uptake by intact wheat plants as related to growth rate. Physiol Plant 72:121–126

  40. Roy J, Navas ML, Sonié L (1989) Invasion by annual bromes: a case study challenging the homoclines approach of invasions. In: Groves RH, di Castri F (eds) Biogeography of mediterranean invasions. Cambridge University Press, Cambridge (in press)

  41. Ruget F (1981) Respiration de croissance et respiration d'entretien: méthodes de mesure, comparaison des résultats. Agronomie 1:601–610

  42. Schulze E-D, Koch G, Percival F, Mooney HA (1985) The nitrogen balance of Raphanus sativus x raphanistrum plants. I. Daily nitrogen use under high nitrate supply. Plant Cell Env 8:713–720

  43. Troughton A (1977) Relationship between the root and shoot systems of grasses. In: Marshall JK (ed) The belowground ecosystem: a synthesis of plant-associated processes. Rge Sc Dpt Science Series no 26, Colorado State University, Fort Collins, pp 39–51

  44. Van de Dijk SJ (1980a) Two ecologically distinct subspecies of Hypochaeris radicata L. I. Nitrate uptake and nitrate utilization. Plant Soil 56:465–473

  45. Van de Dijk SJ (1980b) Two ecologically distinct subspecies of Hypochaeris radicata L. II. Growth response to nitrate and ammonium, growth strategy and formative aspects. Plant Soil 57:111–122

  46. Van de Dijk SJ, Lanting L, Lambers H, Posthumus F, Stulen I, Hofstra R (1982) Kinetics of nitrate uptake by different species from nutrient-rich and nutrient-poor habitats as affected by the nutrient supply. Physiol Plant 55:103–110

  47. Van den Honert TH, Hooymans JJM (1955) On the absorption of nitrate by maize in water culture. Acta Bot Neerl 4:376–384

  48. Wilkinson GM (1961) Statistical estimations in enzyme kinetics. Biochem J 80:324–332

  49. Woldendorp JW (1983) The relation between the nitrogen metabolism of Plantago species and the characteristics of the environment. In: Lee JA, McNeil S, Rorison IH (eds) Nitrogen as an ecological factor. Blackwell Scientific Publications, Oxford, pp 137–166

  50. Yoshida S, Navasero SA, Ramirez EA (1969) Effects of silica and nitrogen supply on some leaf characters of the rice plant. Plant Soil 31:48–56

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Correspondence to E. Garnier or J. Roy.

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Garnier, E., Koch, G.W., Roy, J. et al. Responses of wild plants to nitrate availability. Oecologia 79, 542–550 (1989). https://doi.org/10.1007/BF00378673

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Key words

  • Bromus
  • Relative growth rate
  • Nitrate uptake
  • Limiting external concentration
  • Grasses