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Canine bone marrow cytology as a means of supporting peripheral blood changes

Thrornbocytopenia During the Course of a Toxicity Study

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Abstract

The origin of induced changes in the peripheral blood frequently resides in the bone marrow. Although interim (within study) canine bone marrow analysis provides crucial and vital information supplementary to such changes, it is not a routine technique in normal toxicity studies. A two-tiered approach is reasonable and scientifically justified, with haemocytometry as the first tier, and interim bone marrow analysis as the second, only to be performed if indicated by peripheral blood values.

Additionally, ethical and economic reasons play a decisive role in not performing interim canine marrow sampling on a routine basis, but only on selected occasions. Such an occasion would be the presence of a thrombocytopenia, where an objective assessment of megakaryoblast/megakaryocyte numbers and morphology would be a distinct advantage in determining the aetiology of the condition. However, such evaluations are normally hampered by the relatively small numbers of these cells, and a method for overcoming this problem is described.

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Authors and Affiliations

  1. NV Organon, Department of Drug Safety, PO Box 20, 5340 BH, Oss, The Netherlands

    E. van Esch, P. Salemink, M. Roelofs, M. Steenhof, H. Botermans & D. Dijkhoff

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  1. E. van Esch
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  2. P. Salemink
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  3. M. Roelofs
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  4. M. Steenhof
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  5. H. Botermans
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  6. D. Dijkhoff
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van Esch, E., Salemink, P., Roelofs, M. et al. Canine bone marrow cytology as a means of supporting peripheral blood changes. Comparative Haematology International 6, 242–245 (1996). https://doi.org/10.1007/BF00378118

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  • DOI: https://doi.org/10.1007/BF00378118

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