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Functional requirements of (Phe, G)-A--L-specific T-cell clones of (H-2 b x H-2 kF1 origin

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Abstract

T-cell clones specific for the synthetic polypeptide antigen poly(LPhe, LGlu)-poly(DLAla)--poly(LLys) of (C57BL/6 x C3H/HeJ)F1 origin were tested for their biological activities. One group of clones was restricted in its proliferative response to the H-2 b haplotype, the second to the H-2 k haplotype, and the third to the F1 unique Ia determinants. All the clones which proliferated in response to antigen secreted interleukin-2 (IL-2) following stimulation. The H-2 restriction of the IL-2 secretion was the same as that of the proliferation. Two of the clones tested, C.6 and C.10, could provide help to B cells in antibody production. However, the genetic restriction profile of the helper activity was less stringent than that for the proliferative response. Thus, C.6, which proliferated in the presence of F1 antigen-presenting cells only, could help B cells and accessory cells of C3H/HeJ. C.10, which was restricted in its proliferative response to the H-2 b haplotype, could collaborate with B cells and accessory cells of the H-2 k haplotype as well. The antibody response of both clones was restricted to the parental or F1 strains.

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Abbreviations

(T, G)-A-L:

poly-(LTyr, LGlu)poly(DLAla)--poly(LLys)

(Phe, G)-A--L:

poly(LPhe, LGlu)-poly(DLAla)--poly(LLys)

APC:

antigen-presenting cells

Con A:

concanavalin A

FCS:

fetal calf serum

IL-2:

interleukin-2

References

  1. Apte, R. N., Lowy, I., DeBaetselier, P., and Mozes, E.: Establishment and characterization of continuous helper T cell lines specific to poly (LTyr, LGlu)-poly (DLAla)--poly (LLys). J. Immunol. 127: 25–30, 1981

  2. Asano, Y., Shigeta, M., Fathman, C. G., Singer, A., and Hodes, R. J.: Role of the major histocompatibility complex in T cell activation of B cell subpopulations. A single monoclonal T helper cell population activates different B cell subpopulations by distinct pathways. J. Exp. Med. 156: 350–360, 1982

  3. Axelrod, O. and Mozes, E.: Analysis of the biological functions and fine specificity of (T, G)-A--L specific T cell clone. Immunobiology, in press, 1986

  4. Eshhar, Z., Strassmann, G., Waks, T., and Mozes, E.: In vitro and in vivo induction of effector T cells mediating DTH responses to protein and synthetic polypeptide antigen. Cell. Immunol. 47: 378–389, 1979

  5. Fathman, C. G. and Kimoto, M.: Studies utilizing marine T cell clones: It genes, Ia antigens and MLR stimulating determinants. Immunol. Rev. 54: 57–79, 1981

  6. Fuchs, S. and Sela, M.: Antigenicity of some new synthetic polypeptides and polypeptidyl gelatins. Biochem. J. 93: 566–572, 1964

  7. Gillis, S. and Smith, K. A.: Long term cultures of tumor specific cytotoxic T cells. Nature 268: 154–156, 1977

  8. Kimoto, M. and Fathman, C. G.: Antigen reactive T cell clones. I. Transcomplementing hybrid I-A region gene products function effectively in antigen presentation. J. Exp. Med. 152: 759–770, 1980

  9. Kimoto, M. and Fathman, C. G.: Antigen reactive T cell clones. II. Unique homozygous and (high responder x low responder)F1 hybrid antigen presenting determinants detected using poly (Tyr, Glu)-poly D,L-Ala--poly Lys reactive T cell clones. J. Exp. Med. 153: 375–385, 1981

  10. Koch, N., Hämmerling, G. J., Tada, N., Kimura, S., and Hämmerling, U.: Cross-blocking studies with monoclonal antibodies against I-A molecules of haplotypes b, d and k. Eur. J. Immunol. 12: 909–914, 1982

  11. Mage, M. G., McHugh, L. L., and Rothstein, T. L.: Mouse lymphocytes with and without surface immunoglobulin: Preparative scale dishes coated with specifically purified anti-immunoglobulin. J. Immunol. Methods 15: 47–56, 1977

  12. Mosier, D. E. and Feeney, A. J.: Interaction of helper T cells and Lyb 5+ B cells responding to phosphorylcholine is MHC restricted. J. Exp. Med. 160: 329–334, 1984

  13. Ramila, G., Studer, S., Mischler, S., and Erb, P.: Evaluation of la+ tumor cell lines and peritoneal exudate macrophage as accessory cells: Differential requirements for the activation of certain T cell functions. J. Immunol. 131: 2714–2734, 1983

  14. Reske-Kunz, A. B. and Rude, E.: Insulin specific T cell hybridomas derived from (H-2b x H-2k)F1 mice preferably employ F1 unique restriction elements for antigen recognition. Eur. J. Immunol. 15: 1048–1054, 1985

  15. Schreier, M. H., Andersson, J., Lernhardt, W., and Melchlers, F.: Antigen-specific T-helper cells stimulate H-2 compatible and H-2 incompatible B-cell blasts polyclonally. J. Exp. Med. 151: 194–203, 1980

  16. Sredni, B. and Schwartz, R. H.: Antigen-specific, proliferating T lymphocyte clones. Methodology, specificity, MHC restriction and alloreactivity. Immunol. Rev. 54: 187–223, 1981

  17. Sredni, B., Matis, L. A., Lerner, E. A., Paul, W. E., and Schwartz, R. H.: Antigen specific T cell clones restricted to unique F1 major histocompatibility determinants. Inhibition of proliferation with monoclonal anti-Ia antibody. J. Exp. Med. 153: 677–693, 1981

  18. Waters, S. J., Luzzatti, P. R., and Bona, C. A.: Functional properties of T cell clones with a double specificity for alloantigens and foreign antigens. J. Exp. Med. 160: 1300–1315, 1984

  19. Widera, G. and Flavell, R. A.: The nucleotide sequence of the routine response gene: Evidence for gene conversion events in class II genes of the major histocompatibility complex. EMBO J. 3: 1221–1225, 1984

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Correspondence to Edna Mozes.

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Axelrod, O., Mozes, E. Functional requirements of (Phe, G)-A--L-specific T-cell clones of (H-2 b x H-2 kF1 origin. Immunogenetics 24, 386–390 (1986). https://doi.org/10.1007/BF00377957

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Keywords

  • Polypeptide
  • Antibody Response
  • Antibody Production
  • Proliferative Response
  • Genetic Restriction