, Volume 69, Issue 2, pp 288–295 | Cite as

The analysis of photosynthesis in air and water of Ascophyllum nodosum (L.) Le Jol.

  • Andrew M. Johnston
  • John A. Raven
Original Papers


The photosynthetic characteristics for the intertidal macroalga Ascophyllum nodosum were examined in air and water. Under ambient conditions of temperature (10° C) inorganic carbon concentrations (15.63 mmol CO2 m-3 or 2.0 mol TIC m-3) and light (500 μmol photons m-2 s-1) photosynthesis was slightly greater by the exposed alga than by the submerged alga. In both environments photosynthesis was light saturated at 200 μmol photons m-2 s-1. The relationship between CO2 concentration and photosynthesis in air could be accurately analysed using Michaelis-Menten kinetics, although the range of concentrations used were not saturating. In contrast the application of the Lineweaver-Burk and Woolf plots to aquatic photosynthesis was not suitable as the experimental data was similar to the Blackman type curves and not rectangular hyperbolae. This was reflected by the applicability of the Hill-Whittingham equation to describe the photosynthesis curves. The effect of unstirred layers and other limiting factors is discussed in relation to the kinetic parameters, Vmax and Km.


Photosynthesis Ambient Condition Carbon Concentration Inorganic Carbon Type Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Baardseth E (1970) Synopsis of biological data on knobbed wrack Ascophyllum nodosum (Linnaeus) Le Jolis. F.A.O. Fisheries synopsis, 38Google Scholar
  2. Badger MR (1980) Kinetic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase from Anabaena variabilis. Arch Biochem Biophys 201:247–254Google Scholar
  3. Beer S, Eshel A (1983) Photosynthesis of Ulva sp. II. Utilization of CO2 and HCO3 when submerged. J Exp Mar Biol 70:99–106Google Scholar
  4. Besford RT (1984) Some properties of ribulose bisphosphate carboxylase extracted from tomato leaves. J Exp Bot 35:495–504Google Scholar
  5. Bidwell RGS, McLachlan J (1985) Carbon nutrition of seaweeds: Photosynthesis, photorespiration and respiration. J Exp Mar Biol Ecol 86:15–46Google Scholar
  6. Bird IF, Cornelius MJ, Keys AJ (1980) Effect of carbonic acid on the activity of ribulose bisphosphate carboxylase. J Exp Bot 31:365–369Google Scholar
  7. Blackman FF, Smith AM (1911) Experimental researches on vegetable assimilation and respiration. IX. On assimilation in submerged water plants, and its relation to the concentration of carbon dioxide and other factors. Proc R Soc, Series B 83:389–412Google Scholar
  8. Brechignac F, Andre M (1984) Oxygen uptake and photosynthesis of the Red macroalga, Chondrus crispus, in seawater. Plant Physiol 75:919–923Google Scholar
  9. Brinkhuis BH, Tempel NR, Jones RF (1976) Photosynthesis and respiration of exposed salt-marsh Fucoids. Mar Biol 34:349–359Google Scholar
  10. Chock JS, Mathieson AC (1979) Physiological ecology of Ascophyllum nodosum (L.) Le Jolis and its detached Ecad scorpiodes (Hornemann) Hauck (Fucales, Phaeophyta). Botan Marina 22:21–26Google Scholar
  11. Dietz K, Neimanis S, Heber U (1984) Rate limiting factors in leaf photosynthesis. II. Electron transport. Biochim Biophys Acta 767:444–450Google Scholar
  12. Dromgoole FI (1978) The effects of pH and inorganic carbon on photosynthesis and dark respiration of Carpophyllum (Fucales, Phaeophyceae). Aquatic Bot 4:11–22Google Scholar
  13. Farquhar GD, Caemmerer S von, Berry JA (1980) A biochemical model of photosynthetic CO2 assimilation in leaves of C3 plants. Planta 149:78–90Google Scholar
  14. Hill R, Whittingham CP (1955) Photosynthesis. Methuen and Co. Ltd., LondonGoogle Scholar
  15. Himmelblau DM (1964) Diffusion of dissolved gases in liquids. Chem Rev 64:527–550Google Scholar
  16. Jeffery SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c, and c2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanz 167:191–194Google Scholar
  17. Johnson WS, Gigon A, Gulmon SL, Mooney HA (1974) Comparative photosynthetic capacities of intertidal algae under exposed and submerged conditions. Ecology 55:450–453Google Scholar
  18. Jolliffe PA, Tregunna EB (1970) Studies on HCO3 ion uptake during photosynthesis in benthic marine algae. Phycologia 9:293–303Google Scholar
  19. Jordan DB, Ogren WL (1981) Species variation in the specificity of ribulose bisphosphate carboxylase/oxygenase. Nature 291:513–515Google Scholar
  20. Jordan DB, Ogren WL (1983) Species variation in the kinetic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 227:425–433Google Scholar
  21. Jordan DB, Orgen WL (1984) The CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase. Dependence on ribulose bisphosphate concentration, pH and temperature. Planta 161:308–313Google Scholar
  22. Kanwisher JW (1966) Photosynthesis and respiration in some seaweeds. In: Some contemporary studies in marine sciences, Barnes H (ed). Allen and Unwin, London, pp 407–420Google Scholar
  23. Kaplan A, Badger MR, Berry JA (1980) Photosynthesis and the intracellular inorganic carbon pool in the blue green alga Anabaena variabilis: Response to external CO2 concentration. Planta 149:219–226Google Scholar
  24. Kerby NW, Raven JA (1985) Transport and fixation of inorganic carbon by marine algae. Adv Bot Res 11:71–123Google Scholar
  25. Kremer BP (1981) Carbon metabolism. In: The biology of seaweeds. Lobban CS, Wynne MJ (eds). Blackwell Scientific Publications, London, pp 493–533Google Scholar
  26. Lilley RMcC, Walker DA (1975) Carbon dioxide assimilation by leaves, isolated chloroplasts, and ribulose bisphosphate carboxylase from spinach. Plant Physiol 55:1087–1092Google Scholar
  27. Lloyd NDH, McLachlan JL, Bidwell RGS (1981) A rapid infra-red carbon dioxide analysis screening technique for predicting growth and productivity. Xth International Seaweed Symposium, 461–466Google Scholar
  28. Lucas WJ (1975) Photosynthetic fixation of 14carbon by internodal cells of Chara corallina. J Exp Bot 26:331–346Google Scholar
  29. Lüning K (1981) Light. In: The biology of seaweeds. Lobban CS, Wynne MC (eds) Blackwell Scientific Publications, London, pp 326–355Google Scholar
  30. MacFarlane J (1985) Diffusion and the uptake of nutrients by aquatic macrophytes. PhD Thesis, University of AdeliadeGoogle Scholar
  31. MacFarlane J, Raven JA (1985) External and internal CO2 transport in Lemanea: Interactions with the kinetics of ribulose bisphosphate carboxylase. J Exp Bot 36:610–622Google Scholar
  32. Ogata E, Matsui T (1965) Photosynthesis in several marine plants of Japan in relation to carbon dioxide supply, light and inhibitors. Jap J Bot 19:83–98Google Scholar
  33. Quadir A, Harrison PJ, Wreedie RE de (1979) The effects of emergence and submergence on the photosynthesis and respiration of marine macrophytes. Phycologia 18:83–88Google Scholar
  34. Ramus J, Lemons F, Zimmerman C (1977) Adaptation of lightharvesting pigments to downwelling light and the consequent photosynthetic preformance of the eulittoral rockweeds Ascophyllum nodosum and Fucus vesiculosus. Mar Biol 42:293–303Google Scholar
  35. Raven JA, Smith FA (1980) The chemiosmotic viewpoint. In: Plant membrane transport: Current conceptual issues. Spanswick RM, Lucas WJ, Dainty J (eds). Elsevier/North-Holland Biomedical Press, Amsterdam, pp 161–175Google Scholar
  36. Sand-Jensen K, Gordon DM (1984) Differential ability of marine and freshwater macrophytes to utilize HCO3 and CO2. Mar Biol 80:247–253Google Scholar
  37. Schonbeck MW, Norton TA (1979) An investigation of drought avoidance in intertidal fucoid algae. Botan Marina 22:133–144Google Scholar
  38. Seybold A, Egle K (1937) Quantitative Untersuchungen über die Chlorophylle und Carotinoide der Meeresalgen. Jahrb Wiss Bot 84:50–80Google Scholar
  39. Smith FA, Walker NA (1980) Photosynthesis by aquatic plants: Effects of unstirred layers in relation to assimilation of CO2 and HCO3 and to carbon isotope discrimination. New Phytol 86:245–259Google Scholar
  40. Spalding MH, Ogren WL (1983) Evidence for a saturable transport component in the inorganic carbon uptake of Chlamydomonas reinhardtii. FEBS lett 154:335–338Google Scholar
  41. Wheeler WN (1980) Effect of boundary layer transport on the fixation of carbon by the giant kelp Macrocystis pyriferd. Mar Biol 56:103–110Google Scholar
  42. Yeoh H, Badger MR, Watson L (1981) Variations in kinetic properties of ribulose-1,5-bisphosphate carboxylases among plants. Plant Physiol 67:1151–1155Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Andrew M. Johnston
    • 1
  • John A. Raven
    • 1
  1. 1.Department of Biological SciencesDundee UniversityDundeeUK

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