Roux's archives of developmental biology

, Volume 196, Issue 4, pp 243–247 | Cite as

Haemocytes accumulate collagen transcripts during Drosophila melanogaster metamorphosis

  • Bernard Knibiehler
  • Christian Mirre
  • Jean -Pierre Cecchini
  • Yannick Le Parco
Article

Summary

We report a direct examination of the expression of one collagen gene (DCg1) during Drosophila melanogaster metamorphosis, based on data from in situ hybridization. The transcripts of this gene, thought to encode a basement membrane type IV collagen, are mainly accumulated during ecdysis in wandering haemocytes. Our results demonstrate that haemocytes contribute to extracellular matrix deposition and seem to perform a fibroblastic function during Drosophila development.

Key words

Drosophila Collagen Haemocytes Basement membranes 

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References

  1. Ashhurst DE (1979) Hemocytes and connective tissue: a critical assessment. In: Gupta AP (ed) Insect hemocytes: development, form, functions and techniques. Cambridge University Press, CambridgeGoogle Scholar
  2. Ashhurst DE, Costin NM (1974) The development of a collagenous connective tissue in the locust, Locusta migratoria. Tissue Cell 6:279–300Google Scholar
  3. Bairati A (1964) L'ultrastruttura dell'organo dell'emolinfa nella larva di Drosophila melanogaster. Z Zellforsch 61:769–802Google Scholar
  4. Crossley AC (1975) The cytophysiology of insect blood. In: Treherne JE, Berridge MJ, Wigglesworth VB (eds). Advances in insect physiology, vol 11. Academic Press, New York, pp 117–221Google Scholar
  5. De Biasi S, Pilotto F (1976) Ultrastructural study of collagenous structures in some diptera. J Submicrosc Cytol 8:337–345Google Scholar
  6. El Shatoury HH (1955) The structure of the lymph-glands of Drosophila larvae. Wilhelm Roux's Arch 147:489–495Google Scholar
  7. El Shatoury HH, Waddington CH (1957) Functions of the lymph-gland cells during the larval period in Drosophila. J Embryol Exp Morphol 5:122–133Google Scholar
  8. François J (1985) The collagen of arthropods. In: Bairati A, Garrone R (eds) Biology of invertebrate and lower vertebrate collagens. Plenum Press, New York, pp 345–368Google Scholar
  9. Hafen E, Levine M, Garber RL, Gehring WJ (1983) An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic antennapedia gene complex. EMBO J 2:617–623Google Scholar
  10. Hayashi S, Gilliam JC, Delancy AD, Tener GM (1978) Acetylation of chromosome squashes of Drosophila melanogaster decreases the background of autoradiographs from hybridization with 125I-labelled RNA. J Histochem Cytochem 26:677–679Google Scholar
  11. Hoffmann JA (1970) Les organes hematopoïetiques de deux insectes orthoptères: Locusta migratoria et Gryllus bimaculatus. Z Zellforsch 106:451–472Google Scholar
  12. Jones JC (1962) Current concepts concerning insect hemocytes. Ann Zool 2:209–246Google Scholar
  13. Lawrence PA, Johnston P (1986) Observations on cell lineage of internal organs of Drosophila. J Embryol Exp Morphol 91:251–266Google Scholar
  14. Le Parco Y, Knibiehler B, Cecchini JP, Mirre C (1986a) Stage and tissue-specific expression of a collagen gene during Drosophila melanogaster development. Exp Cell Res 163:405–412Google Scholar
  15. Le Parco Y, Cecchini JP, Knibiehler B, Mirre C (1986b) Characterization and expression of collagen-like genes in Drosophila melanogaster. Biol Cell 56:217–226Google Scholar
  16. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbour LaboratoryGoogle Scholar
  17. Monson JM, Natzle JE, Friedman J, McCarthy BJ (1982) Expression and novel structure of a collagen gene in Drosophila. Proc Natl Acad Sci 79:1761–1765Google Scholar
  18. Natzle JE, Monson JM, McCarthy BJ (1982) Cytogenetic location and expression of collagen-like gene in Drosophila. Nature (Lond) 296:368–371Google Scholar
  19. Riski TM (1978) The circulatory system and associated cells and tissues. In: Ashburner M, Wright TRF (eds) The genetics and biology of Drosophila. Academic Press, New York, pp 397–451Google Scholar
  20. Robertson CW (1936) The metamorphosis of Drosophila melanogaster including an accurately timed account of the principal morphological changes. J Morphol 59:351–399Google Scholar
  21. Scharrer B (1972) Cytophysiological features of hemocytes in cockroaches. Z Zellforsch Mikrosk Anat 129:301–319Google Scholar
  22. Whitten JM (1962) Breakdown and formation of connective tissue in the pupal stage of an insect. Q J Microsc Sci 103:359–367Google Scholar
  23. Whitten JM (1964) Haemocytes and the metamorphosing tissues in the pupal stage of an insect. Q J Microsc Sci 103:359–367Google Scholar
  24. Whitten JM (1964) Haemocytes and the metamorphosing tissues in Sarcophaga bullata, Drosophila melanogaster and other cyclorrhaphous diptera. J Insect Physiol 10:447–469Google Scholar
  25. Wigglesworth VB (1973) Haemocytes and basement membrane formation in Rhodnius. J Insect Physiol 19:831–844Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Bernard Knibiehler
    • 1
  • Christian Mirre
    • 1
  • Jean -Pierre Cecchini
    • 1
  • Yannick Le Parco
    • 1
  1. 1.Faculté des Sciences de LuminyLaboratoire de Biologie de la Différenciation CellulaireMarseille Cedex 9France

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