Immunogenetics

, Volume 27, Issue 4, pp 288–292 | Cite as

Mouse immunoglobulin allotypes: multiple differences between the nucleic acid sequences of the IgE a and IgE b alleles

  • Yoichi Shinkai
  • Hiromitsu Nakauchi
  • Tasuku Honjo
  • Ko Okumura
Article
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Abstract

To clarify the allotypic difference of the IgE antibody molecule, we determined the complete nucleotide sequence of the genes encoding the constant portion of mouse IgE of a (BALB/c) as well as b (B10.A) allotypes. A comparison of the sequences revealed that there were 12 single-base changes: 2 single-base changes in CH1 and CH2, 3 in CH3, and 7 in CH4. Five of them were silent changes, but seven resulted in amino acid substitutions. Although the silent changes are scattered through CH1 to CH4, the nonsilent substitutions were found only in CH3 (two substitutions) and CH4 (five). The allotypic determinant(s) that conventional antisera detect most likely reflects an amino acid difference(s) in CH3 and/or CH4.

Keywords

Nucleotide Nucleic Acid Nucleotide Sequence Amino Acid Substitution Complete Nucleotide Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Yoichi Shinkai
    • 1
  • Hiromitsu Nakauchi
    • 2
  • Tasuku Honjo
    • 3
  • Ko Okumura
    • 1
  1. 1.Department of ImmunologyJuntendo University School of MedicineTokyoJapan
  2. 2.Laboratory of Molecular Regulation of Aging, Frontier Research ProgramTsukuba Life Science Center, RIKEN, The Institute of Physical and Chemical ResearchIbarakiJapan
  3. 3.Department of Medical ChemistryKyoto University Faculty of MedicineKyotoJapan

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