Archives of Dermatological Research

, Volume 284, Issue 4, pp 238–241 | Cite as

Measurement of S phase duration in human epidermis using cyclin immunostaining and 3H-thymidine pulse labelling

  • E. Berlingin
  • M. Heenen
  • P. Galand
Original Contributions
Received:

Summary

Cyclin/PCNA (proliferating cell nuclear antigen) is a nuclear protein strongly associated with DNA replication sites. Under methanol fixation, cyclin immunostaining might serve as a specific marker for S-phase cells. Associated with single labelling with 3H-thymidine, this immunodetection represents an easy method for measuring S-phase duration. Its application to six samples of normal human epidermis allowed us to measure an S phase of 9.7±0.3 h, a result in fair agreement with a previous estimate of 10.2 h obtained from a classical doublelabelling technique with two different concentrations of 3H-thymidine.

Key words

S phase Cyclin immunostaining 3H-thymidine pulse labelling 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • E. Berlingin
    • 1
  • M. Heenen
    • 1
  • P. Galand
    • 2
  1. 1.Department of DermatologyHospital Erasme, Free University of BrusselsBrusselsBelgium
  2. 2.Laboratory of Cytology and Experimental Cancerology and Biology UnitInstitute for Interdisciplinary Research, School of Medicine, Free University of BrusselsBelgium

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