Pflügers Archiv

, Volume 423, Issue 5–6, pp 511–518

Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy

  • G. J. Stuart
  • H. U. Dodt
  • B. Sakmann
Molecular and Cellular Physiology

Abstract

A description is given of the implementation of infrared differential interference contrast (IR-DIC) video microscopy to an upright compound microscope. Using the improved resolution offered by IR-DIC a procedure is described for making patch-pipette recordings from visually identified neuronal somata and dendrites in brain slices. As an example of the application of this technique to electrophysiological recordings from small neuronal processes in brain slices we describe wholecell current-clamp and cell-attached and excised patchclamp recordings from the apical dendrites of layer V pyramidal neurons in slices of rat neocortex.

Key words

Patch clamp Brain slice Dendrite Video microscopy Neocortex 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • G. J. Stuart
    • 1
  • H. U. Dodt
    • 2
  • B. Sakmann
    • 1
  1. 1.Abteilung ZellphysiologieMax-Planck-Institut für medizinische ForschungHeidelbergGermany
  2. 2.Klinisches InstitutMax-Planck-Institut für PsychiatrieMünchen 40Germany

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