Pflügers Archiv

, Volume 421, Issue 2–3, pp 131–137 | Cite as

Inhibition of voltage-dependent Ca2+ channels via α2-adrenergic and opioid receptors in cultured bovine adrenal chromaffin cells

  • Thomas Kleppisch
  • Gudrun Ahnert-Hilger
  • Maik Gollasch
  • Karsten Spicher
  • Jürgen Hescheler
  • Günter Schultz
  • Walter Rosenthal
Excitable Tissues and Central Nervous Physiology
Received:
Revised:
Accepted:

Abstract

Adrenal chromaffin cells secrete catecholamindes and opioids. The effects of these agents on whole-cell Ca2+ channel currents were studied, using bovine adrenal chromaffin cells kept in short term culture. Ca2+ channel currents recorded during voltageclamp pulses from a holding potential of −80 mV to 0 mV were reversibly reduced by 10 μM epinephrine (in the presence of 1 μM propranolol) or 5 μM of the synthetic opioid, d-Ala2-d-Leu5-enkephalin (DADLE) by approximately 35% and 25%, respectively. The inhibitory action of epinephrine was mimicked by clonidine, reduced by yohimbine but not affected by prazosin. The DADLE-induced reduction of the Ca2+ channel current was antagonized by naloxone. The dihydropyridine (+)PN 200-110 (5 μM) reduced the Ca2+ channel current by approximately 40%; the Ca2+ channel current inhibited by (+)PN 200-110 was not further reduced by epinephrine. Intracellular infusion of guanosine-5′-O-(2-thiodiphosphate) and pretreatment of cells with pertussis toxin abolished the inhibitory effect of both epinephrine and DADLE. In membranes of adrenal chromaffin cells, four pertussis-toxin-sensitive G-proteins were identified, including Gi1, Gi2, Go1 and another Go subtype, possibly Go2. The data show that activation of α2-adrenergic and opioid receptors causes an inhibition of dihydropyridine-sensitive Ca2+ channels in adrenal chromaffm cells. These inhibitory modulations are mediated by pertussis-toxin-sensitive G-proteins and may represent a mechanism for a negative feedback signal by agents released from the adrenal medulla.

Key words

Adrenal chromaffin cell Voltage-dependent Ca2+ channels α2-Adrenoceptors Opioid receptors G-proteins Patch-clamp 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Thomas Kleppisch
    • 1
  • Gudrun Ahnert-Hilger
    • 3
  • Maik Gollasch
    • 1
  • Karsten Spicher
    • 2
  • Jürgen Hescheler
    • 2
  • Günter Schultz
    • 2
  • Walter Rosenthal
    • 2
  1. 1.Institut für PhysiologieHumboldt-Universität zu BerlinBerlinFederal Republic of Germany
  2. 2.Institut für PharmakologieFreie Universität BerlinBerlin 33Federal Republic of Germany
  3. 3.Institut für NeuropsychopharmakologieFreie Universität BerlinBerlin 33Federal Republic of Germany

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