Pflügers Archiv

, Volume 426, Issue 1–2, pp 171–173 | Cite as

Intracellular calcium modulates basolateral K+-permeability in frog skin epithelium

  • Birger Brodin
  • Klaus Asger Rytved
  • Robert Nielsen
Short Communication Molecular and Cellular Physiology
Received:
Revised:
Accepted:

Abstract

Cytosolic calcium ([Ca2+]i) has been suggested as a key modulator in the regulation of active sodium transport across electrically “tight” (high resistance) epithelia. In this study we investigated the effects of calcium on cellular electrophysiological parameters in a classical model tissue, the frog skin. [Ca2+]i was measured with fura-2 in an epifluorescence microscope setup. An inhibition of basolateral potassium permeability was observed when cytosolic calcium was increased. This inhibition was reversible upon removal of calcium from the serosal solution.

Key words

K+ channel intracellular calcium thapsigargin fura-2 microelectrode -tight epithelium 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Birger Brodin
    • 1
  • Klaus Asger Rytved
    • 1
  • Robert Nielsen
    • 1
  1. 1.Laboratory of Biological Chemistry A, August Krogh InstituteUniversity of CopenhagenCopenhagenDenmark

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