Pflügers Archiv

, Volume 430, Issue 1, pp 148–150 | Cite as

Ca2+-activated K+-channels in the nuclear envelope isolated from single pancreatic acinar cells

  • Yoshio Maruyama
  • Hitoshi Shimada
  • Junichi Taniguchi
Short Communication Molecular and Cellular Physiology


The patch-clamp techniques are applied to the outer membrane of the nuclear envelope isolated from rat pancreatic acinar cells. The nucleus identified under an inverted microscope was removed by cell surgery from enzymatically dispersed single cells. All the patch-clamp techniques, in situ, excised, and whole-material recordings were applied to the envelope. We have found voltage- and Ca2+-activated K+-channels with an unitary conductance of 200 pS in the outer membrane. The channels are activated by lumen positive potentials and by an increase in luminal Ca2+ concentration. They may play a role for controlling Ca2+-release from the lumen of the nuclear envelope (endoplasmic reticulum) to the nucleoplasm and the perinuclear cytoplasm.

Key words

Nuclear envelope Patch-clamp technique Ca2+-activated K+-channels Pancreatic acinar cells 


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Copyright information

© Springer Verlag 1995

Authors and Affiliations

  • Yoshio Maruyama
    • 1
  • Hitoshi Shimada
    • 2
  • Junichi Taniguchi
    • 3
  1. 1.Department of PhysiologyJichi Medical SchoolTochigiJapan
  2. 2.Department of AnesthesiologyGumma University School of MedicineGummaJapan
  3. 3.Department of PharmacologyJichi Medical SchoolTochigiJapan

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