Isolation of human skin-derived lymph: flow and output of cells following sodium lauryl sulphate-induced contact dermatitis
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By means of microsugery a peripheral subcutaneous lymph vessel draining a defined skin area was isolated and cannulated on the lower leg of six healthy volunteers. Lymph was collected over a period of 8 days. During the first 2 days baseline values for lymph flow and output of cells were established. A contact dermatitis was then induced in the drained skin area by the application of 10% sodium lauryl sulphate. All six probands developed a mild to moderate irritant contact dermatitis. Lymph flow as well as output of cells increased with the intensity of the skin reaction. Subsequent local treatment with clobetasol propionate decreased the cell output, but the lymph flow increased further. Neither lymph flow nor output of cells returned to the initial baseline values at the end of the study, when the clinical signs of contact dermatitis had completely disappeared. During the experiment significant individual variations were found, with means ranging from 0.10 to 0.48 ml/h for lymph flow and from 8700 to 174000/h for cells, which probably depended mainly on the different topographies and calibres of the cannulated lymph vessels.
Key wordsSkin-derived lymph Sodium lauryl sulphate Contact dermatitis
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- 1.Cua AB, Wilhelm KP, Maibach HI (1990) Cutaneous sodium lauryl sulphate irritation potential: age and regional variability. Br J Dermatol 123: 607–613Google Scholar
- 2.Engeset A, Olszewski WL, Jaeger PM, Sokolowski J, Theodorsen L (1977) Twenty-four hour variation in flow and composition of leg lymph in normal men. Acta Physiol Scand 99: 140–148Google Scholar
- 3.Engeset A, Sokolowski J, Olszewski WL (1977) Variation in output of leukocytes and erythrocytes in human peripheral lymph during rest and activity. Lymphology 10: 198–203Google Scholar
- 4.Kripke ML, Munn CG, Jeevan A, Tang JM, Bucana C (1990) Evidence that cutaneous antigen-presenting cells migrate to regional lymph nodes during contact sensitization. J Immunol 145: 2833–2838Google Scholar
- 5.Olszewski WL, Engeset A, Sokolowski J (1977) Lymph flow and protein in the normal male leg during lying, getting up, and walking. Lymphology 10: 178–183Google Scholar
- 6.Olszewski WL, Engeset A, Jaeger PM, Sokolowski J, Theodorsen L (1977) Flow and composition of leg lymph in normal human men during venous stasis, muscular activity and local hyperthermia. Acta Physiol Scand 99: 149–155Google Scholar
- 7.Pflug JJ, Calnan JS (1955) The normal anatomy of the lymphatic system in the human leg. Br J Surg 58: 757Google Scholar
- 8.Ryan TJ, Mortimer PS, Jones RL (1986) Lymphatics of the skin. Neglected but important. Int J Dermatol 25: 411–419Google Scholar
- 9.Willis CM, Stephens CJM, Wilkinson JD (1988) Experimentally-induced irritant contact dermatitis. Contact Dermatitis 18: 20–24Google Scholar