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Immunogenetics

, Volume 17, Issue 4, pp 411–425 | Cite as

Expression of T-lymphoblast-encoded HLA-DR antigens on human T-B lymphoblast hybrids

  • David N. Howell
  • Peter Cresswell
Original Investigations

Abstract

The mode of expression of novel HLA-DR antigens on hybrids of human T and B lymphoblastoid cell lines (LCL) was examined by several approaches. In each case, the results indicated that the novel antigens are T-LCL-encoded. First, hybrids of sublines of the T-LCL CEM with three different B-LCL express indistinguishable sets of novel HLA-DR antigens. Second, the novel HLA-DR and MT specificities of WI-L2 × HSB (a hybrid of a subline of the T-LCL HSB and the B-LCL WI-L2) match those of SB, a B-LCL derived from the same individual as HSB. Finally, an immunoselected variant of SB × CEM.1 (a hybrid of a subline of CEM and SB) lacking one copy of chromosome 6 and one of the hybrid's novel HLA-DR specificities also lacks a class I antigen known to be encoded by CEM.

Keywords

Lymphoblastoid Cell Line 
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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • David N. Howell
    • 1
  • Peter Cresswell
    • 1
  1. 1.Division of ImmunologyDuke University Medical CenterDurham

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