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Pflügers Archiv

, Volume 415, Issue 3, pp 255–261 | Cite as

Direct modulation of voltage-dependent calcium channels by muscarinic activation of a pertussis toxin-sensitive G-protein in hippocampal neurons

  • M. Toselli
  • J. Lang
  • T. Costa
  • H. D. Lux
Excitable Tissues and Central Nervous Physiology

Abstract

Acetylcholine (Ach) reversibly reduces the high voltage-activated (HVA) calcium (Ca) current in hippocampal neurons. Pretreatment of the cells with pertussis toxin (PTX) abolishes the Ach effect, suggesting that PTX-sensitive GTP-binding regulatory proteins (G-proteins) are involved in the signal transduction mechanism that links Ach receptor activation to inhibition of Ca channel activity. This effect is mimicked by intracellular application of the nonhydrolyzable GTP analog GTPγS. Intracellular application of purified G-proteins restored the response to Ach in PTX-treated cells. Furthermore, Ach inhibits the Ca current independently of the presence of cyclic AMP and of the protein kinase C inhibitor H-7 and neither does the Ach effect on the Ca current seem to be correlated to a transient increase in intracellular Ca. Our results suggest that activation of the α-subunit of the PTX-sensitive G-protein could directly modulate the HVA Ca channel without involving second messenger systems.

Key words

Ca channels G-proteins Hippocampus Acetylcholine 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Toselli
    • 1
  • J. Lang
    • 2
  • T. Costa
    • 2
  • H. D. Lux
    • 1
  1. 1.Abteilung NeurophysiologieMax-Planck-Institut für PsychiatriePlaneggFederal Republic of Germany
  2. 2.Abteilung NeuropharmakologieMax-Planck-Institut für PsychiatriePlaneggFederal Republic of Germany

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