Studies on a continuous recycle reactor for a lipase-catalysed processing of ricebran oil
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Abstract
The authors have developed a continuous recycle reactor which efficiently performs emulsion type enzymatic reactions. The reactor column is filled with immobilised lipase and the reactions are effected by pumping the pre-prepared oil-water emulsion through the bottom of the reactor. A part of the product was recycled back and this type of recycling greatly improves the productivity of fatty acid compared to continuous once-through reactor without recycling. The recycle reactor could be continuously run for 35 days without decrease in conversions. The performance of the reactor was interpreted by a model and the theoretical conversion was compared with the experimental data.
Keywords
Experimental Data Waste Water Lipase Water Management Water PollutionList of Symbols
- FAOμmol/min
feed rate
- KM g/l
Michaelis constant
- R
recycle ratio
- r5μmol/(ml · min)
reaction rate
- S0 g/l
initial substrate concentration
- Vmaxμmol/(ml · min)
maximum reaction velocity
- VR l
void volume of the reactor
- xs
fractional conversion
- Standard deviation
\(\left( {{{\left[ {\mathop \Sigma \limits_1^n \left\{ {\left( {C_{exp} - C_{model} } \right)/C_{exp} } \right\}^2 } \right]} \mathord{\left/ {\vphantom {{\left[ {\mathop \Sigma \limits_1^n \left\{ {\left( {C_{exp} - C_{model} } \right)/C_{exp} } \right\}^2 } \right]} {(n - 1)^{0.5} }}} \right. \kern-\nulldelimiterspace} {(n - 1)^{0.5} }}} \right)\)
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References
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