Bioprocess Engineering

, Volume 3, Issue 2, pp 69–72 | Cite as

Reactor design for the ABE fermentation using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar

  • N. Qureshi
  • I. S. Maddox
Originals

Abstract

Cells of Clostridium acetobutylicum were immobilized by adsorption onto bonechar. and used for the production of solvents (ABE fermentation) from whey permeate. When the process was performed in packed bed reactors operated in a vertical or inclined mode, solvent productivities approximating 6 kg/(m3h) were obtained. However, the systems suffered from blockage due to excess biomass production and gas hold-up. These problems were less apparent when a partially-packed bed reactor was operated in the horizontal mode. A fluidized bed reactor proved to be the most stable of the systems investigated, and a productivity of 4.8 kg/(m3h) was maintained over a period of 2000 h of operation. The results demonstrate that this type of reactor may have a useful future role in the ABE fermentation.

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References

  1. 1.
    Toda, K.; Ohtake, H.; Asakura, T.: Ethanol production in horizontal bioreactor. Appl. Microbiol. Biotechnol. 24 (1986) 97–101Google Scholar
  2. 2.
    Shiotani, T.; Yamane, T.: A horizontal packed bed bioreactor to reduce CO2 holdup in the continuous production of ethanol by immobilized yeast cells. Eur. J. Appl. Microbiol. Biotechnol. 13 (1981) 96–101Google Scholar
  3. 3.
    Juneja, L. R.; Terasawa, M.; Yamane, T.; Shimizu, S.: Continuous ethanol fermentation using flocculent yeast entrapped in horizontal parallel flow bioreactor system. Biotechnol. Lett. 8 (1986) 431–436Google Scholar
  4. 4.
    Chotani, G. K.; Constantinides, A.: Immobilized cell cross flow reactor. Biotechnol. Bioeng. 26 (1984) 217–220Google Scholar
  5. 5.
    Krouwel, P. G.; Groot, W. J.; Kossen, N. W. F.; van der Laan, W. F. M.: Continuous isopropanol butanol and ethanol fermentation by immobilized Clostridium beijerinckii cells in a packed bed fermenter. Enzyme Microb. Technol. 5 (1983) 46–54Google Scholar
  6. 6.
    Ennis, B. M.; Maddox, I. S.; Schoutens, G. H.: Immobilized Clostridium acetobutylicum for continuous butanol production from whey permeate. New Zealand J. Dairy Sci. Technol. 21 (1986) 99–109Google Scholar
  7. 7.
    Qureshi, N.; Maddox, I. S.: Continuous solvent production from whey permeate using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Enzyme Microb. Technol. In pressGoogle Scholar
  8. 8.
    Qureshi, N.; Pai, J. S.; Tamhane, D. V.: Reactors for ethanol production using immobilized yeast cells. J. Chem. Technol. Biotechnol. 39 (1987) 75–84Google Scholar
  9. 9.
    Ennis, B. M.; Maddox, I. S.: Use of Clostridium acetobutylicum P262 for production of solvents from whey permeate. Biotechnol. Lett. 7 (1985) 601–606Google Scholar
  10. 10.
    Ennis, B. M.; Qureshi, N.; Maddox, I. S.: In-line toxic product removal during solvent production by continuous fermentation using immobilized Clostridium acetobutylicum. Enzyme Microb. Technol. In pressGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • N. Qureshi
    • 1
  • I. S. Maddox
    • 1
  1. 1.Biotechnology DepartmentMassey UniversityPalmerston NorthNew Zealand

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