Chromosoma

, Volume 81, Issue 3, pp 431–437 | Cite as

A Drosophila melanogaster cell line tested for the presence of active NORs by silver staining

  • Enrica Privitera
Article

Abstract

Silver staining was used to detect active NORs in a Drosophila melanogaster cell line (C1 82) characterized by dimorphic X chromosomes (XXL), one of the two Xs showing a marked increase in heterochromatin where the nucleolar organizer (NO) is located. The Q-banding technique was used to determine the karyotype characteristics of the line. Ag-positive NORs appeared only on structurally changed X chromosomes (XL), both in diploid and tetraploid cells, indicating that rRNA genes of XL are more active or numerous than those on normal homologues. A possible relationship between NOR stainability, the presence of an increased heterochromatic portion and the selective advantage of XXL cells, recurrent in numerous Drosophila female lines, is discussed.

Keywords

Developmental Biology Silver Staining Selective Advantage Nucleolar Organizer Tetraploid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beck, H., Srdić, Ž.: Heterochromatin in mitotic chromosomes of Drosophila hydei. Genetica (Den Haag) 50, 1–10 (1979)Google Scholar
  2. Czaker, R.: Silver staining in transcriptionally active NORs of meiotic and mitotic cells in Acheta domesticus (L.) (Orthoptera). Chromosoma (Berl.) 68, 187–193 (1978)Google Scholar
  3. Dev V.G., Tantravahi, R., Miller, D.A., Miller, O.J.: Nucleolar organizers in Mus museums subspecies and in the RAG mouse cell line. Genetics 86, 389–398 (1977)Google Scholar
  4. Dolfini, S.: Karyotype polymorphism in a cell population of Drosophila melanogaster cultured in vitro. Chromosoma (Berl.) 33, 196–208 (1971)Google Scholar
  5. Echalier, G., Ohanessian, A.: Isolement, en cultures in vitro, de lignees cellulaires diploides de Drosophila melanogaster. C.R. Acad. Sci. (Paris) 268, 1771–1773 (1969)Google Scholar
  6. Echalier, G., Ohanessian, A.: In vitro culture of Drosophila melanogaster embryonic cells. In Vitro 6, 162–172 (1970)Google Scholar
  7. Faccio Dolfini, S., Halfer, C.: Variazioni del cariotipo in popolazioni di cellule di Drosophila coltivate in vitro. Atti dei Convegni Lincei 14, 375–396 (1976)Google Scholar
  8. Goodpasture, C., Bloom, S.E.: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma (Berl.) 53, 37–50 (1975)Google Scholar
  9. Goodpasture, C., Bloom, S.E., Hsu, T.C., Arrighi, F.E.: Human nucleolus organizers: the satellites or the stalks? Amer. J. Hum. Genet. 28, 559–566 (1976)Google Scholar
  10. Halfer, C.: Karyotypic evolution in an originally XY cell line of Drosophila melanogaster. A case of heterochromatin increase in vitro. Chromosoma (Berl.) 68, 149–163 (1978)Google Scholar
  11. Halfer, C., Privitera, E., Barigozzi, C.: A study of spontaneous chromosome variations in seven cell lines derived from Drosophila melanogaster stocks marked by translocations. Chromosoma (Berl.) 76, 201–218 (1980)Google Scholar
  12. Heneen, W.K.: Silver staining of nucleolar organizers and nucleoli during mitosis and interphase of mammalian cells. Genetica (Den Haag) 50, 31–39 (1979)Google Scholar
  13. Howell, W.M.: Visualization of ribosomal gene activity: silver stains proteins associated with rRNA transcribed from oocyte chromosomes. Chromosoma (Berl.) 62, 361–367 (1977)Google Scholar
  14. Hubbell, H.R., Hsu, T.C.: Identification of nucleolar organizer regions (NORs) in normal and neoplastic human cells by the silver staining technique. Cytogenet. Cell Genet. 19, 185–196 (1977)Google Scholar
  15. Hubbell, H.R., Rothblum, L.I., Hsu, T.C.: Identification of a silver binding protein associated with the cytological silver staining of actively transcribing nucleolar regions. Cell. Biol. Int. Rep. 3, 615–622 (1979)Google Scholar
  16. Kakpakov, V.T., Gvozdev, V.A., Platova, T.P., Polukarova, L.G.: In vitro establishment of embryonic cell lines of Drosophila melanogaster. Genetika (USSR) 5, 67–75 (1969)Google Scholar
  17. Martin-DeLeon, P.A., Fleming, M.E., Petrosky, D.L.: Patterns of silver staining in cells of six-day blastocyst and kidney fibroblast of the domestic rabbit. Chromosoma (Berl.) 67, 245–252 (1978)Google Scholar
  18. Miller, D.A., Dev, V.G., Trantavahi, R., Miller, O.J.: Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp. Cell Res. 101, 235–243 (1976)Google Scholar
  19. Pearson, P.L., Bobrow, M., Vosa, C.G.: Technique for identifying Y chromosome in human interphase nuclei. Nature (Lond.) 226, 78–80 (1970)Google Scholar
  20. Ritossa, F.M., Spiegelman, S.: Localization of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 53, 737–745 (1965)Google Scholar
  21. Schwarzacher, H.G., Mikelsaar, A.V., Schnedl, W.: The nature of the Ag-staining of nucleolus organizer regions. Electron- and light-microscopic studies. Cytogenet. Cell Genet. 20, 24–39 (1978)Google Scholar
  22. Tantravahi, R., Miller, D.A., Miller, O.J.: Ag-staining of nucleolar organizer regions of chromosomes after Q-, C-, G- or R-banding procedures. Cytogenet. Cell Genet. 18, 364–369 (1977)Google Scholar
  23. Varley, J.M.: Patterns of silver staining of human chromosomes. Chromosoma (Berl.) 61, 207–214 (1977)Google Scholar
  24. Warburton, D., Atwood, K.C., Henderson, A.S.: Variation in the number of genes for rRNA among human acrocentric chromosomes: correlation with frequency of satellite association. Cytogenet. Cell Genet. 17, 221–230 (1976)Google Scholar
  25. Ward, O.G.: Dimorphic nucleolar organizer regions in the frog Rana blairi. Canad. J. Genet. Cytol. 19, 51–57 (1977)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Enrica Privitera
    • 1
  1. 1.Institute of GeneticsUniversity of MilanoMilanoItaly

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