The ratio of milk-clotting activity to proteolytic activity (MC/PA) was used as an index to determine the quality of milk-clotting enzyme. Solid-state fermentation on wheat bran for 5 days at room temperature gave optimal production for enzyme by Rhizomucor miehei and R. pusillus. A ratio of wheat bran to moisture of 1:0.6 (w/v) gave best results. Adding skim milk powder to the media of R. miehei did not improve the MC/PA ratio but 4% (w/w) of the powder did improve the ratio with R. pusillus. Co-cultivation of R. miehei with R. pusillus did not change the MC/PA ratio.
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The authors are with the Department of Biotechnogy, Bharathiar University. Coimbatore-641 046 India
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Preetha, S., Boopathy, R. Influence of culture conditions on the production of milk-clotting enzyme from Rhizomucor . World J Microbiol Biotechnol 10, 527–530 (1994). https://doi.org/10.1007/BF00367659
- milk-clotting activity
- proteolytic activity
- Rhizomucor miehei
- Rhizomucor pusillus