Veterinary Research Communications

, Volume 15, Issue 5, pp 395–407 | Cite as

Quantitative relationships between suckling-induced teat stimulation and the release of prolactin, gastrin, somatostatin, insulin, glucagon and vasoactive intestinal polypeptide in sows

  • B. Algers
  • A. Madej
  • S. Rojanasthien
  • K. Uvnäs-Moberg
Physiology

Abstract

The pituitary hormones prolactin and oxytocin play important roles in the production and ejection of milk. In addition, some gastrointestinal peptides are released in response to suckling. During suckling, the piglets massage the udder of the sow both before and after let-down and the duration of suckling is correlated to the amount of milk produced by the sow. The aim of this study was to investigate whether there is a quantitative relation between the release of prolactin, gastrin, somatostatin, insulin, glucagon and vasoactive intestinal polypeptide (VIP) and the amount of stimulation of the sow's teats by the piglets.

Repeated blood samples were drawn from three Swedish Landrace sows during three consecutive nursings by each sow on days 1, 3, 7 and 14 after parturition. The duration of massage by the piglets was noted, as was the number of piglets massaging. Hormone levels were quantified by radioimmunoassay.

The releases of prolactin, somatostatin, insulin, glucagon and VIP but not of gastrin were found to be significantly related to the amount of teat massage performed by the piglets during the first 2 weeks of lactation. The release was related to the duration of piglet massage or to the combined effect of duration and the number of piglets massaging but not to the number of piglets massaging per se. The basal level of prolactin was found to decrease during this time.

Keywords

gastrin glucagon hormones insulin nursing pigs prolactin somatostatin suckling VIP vasoactive intestinal polypeptide 

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References

  1. Algers, B. and Jensen, P., 1985. Communication during suckling in the domestic pig. Effects of continuous noise. Applied Animal Behaviour Science, 14, 49–61Google Scholar
  2. Algers, B., Rojanasthien, D. and Uvnäs-Moberg, K., 1990. The relationship between teat stimulation, oxytocin release and grunting rate in the sow during nursing. Applied Animal Behaviour Science, 26, 267–276Google Scholar
  3. Altszuler, N. and Hampshire, J., 1981. Oxytocin infusion increases plasma insulin and glucagon and glucose production and uptake in the normal dog. Diabetes, 30, 112–114Google Scholar
  4. Benjaminsen, E., 1981a. Plasma prolactin in the sow with emphasis on variation in resumption of ovarian activity after weaning. Acta Veterinaria Scandinavica 22, 67–77Google Scholar
  5. Benjaminsen, E., 1981b. Effect of prolactin suppression on the ovarian activity in the lactating sow. Acta Veterinaria Scandinavica, 22, 189–197Google Scholar
  6. Bevers, M.M., Willemse, A.H. and Kruip, ThAM. 1978. Plasma prolactin levels in the sow during lactation and the postweaning period as measured by radioimmunoassay. Biology of Reproduction, 19, 628–634Google Scholar
  7. Blitz, W. and Charbon, G.A., 1983. Regional vascular influences of vasoactive intestinal polypeptide. Scandinavian Journal of Gastroenterology, 18, 755–763Google Scholar
  8. Bolander, F.F., Nicholas, K.R., VanWyk, J.J. and Topper, Y.J. 1981. Insulin is essential for accumulation of casein mRNA in mouse mammary epithelial cells. Proceedings of the National Academy of Sciences of the United States of America, 78, 5682–5684Google Scholar
  9. Cowie, A.T. and Tindal, J., 1971. The Physiology of Lactation, (Edward Arnold Ltd., London), 137–282Google Scholar
  10. Efendic, S., Enzmann, F., Nylén, A., Uvnäs-Wallensten, K. and Luft, R., 1980. Sulphonylurea (Glibenclamide) enhances somatostatin and inhibits glucagon release induced by arginine. Acta Physiologica Scandinavica, 108, 231–233Google Scholar
  11. Eriksson, M., Linden, A. and Uvnäs-Moberg, K., 1987. Suckling increases insulin and glucagon levels in peripheral venous blood of lactating dogs. Acta Physiologica Scandinavica, 131, 391–396Google Scholar
  12. Fahrenkrug, J. and Schaffalitzky de Muckadell, O.B., 1977. Radioimmunoassay of vasoactive intestinal polypeptide (VIP) in plasma. Journal of Laboratory and Clinical Medicine, 80, 1379–1388Google Scholar
  13. Folley, S.J. and Knaggs, G.S., 1966. Milk-ejection activity (oxytocin) in the external jugular vein blood of the cow, goat and sow, in relation to the stimulus of milking or suckling. Journal of Endocrinology, 34, 197–214Google Scholar
  14. Fraser, D.A., 1980. A review of the behavioural mechanism of milk ejection of the domestic pig. Applied Animal Ethology, 6, 247–255Google Scholar
  15. Gill, J.C. and Thomson, W., 1956. Observations on the behaviour of suckling pigs. British Journal of Animal Behaviour, 4, 46–51Google Scholar
  16. Greenwood, F.G., Hunter, W.H. and Glover, J.S., 1963. The preparation of 131I-labelled human growth hormone of high specific radioactivity. Biochemical Journal, 89, 114–123Google Scholar
  17. Gromadzka-Ostrowska, J., Madej, A. and Barcikowski, B., 1985. Peripheral plasma prolactin concentrations during oestrus cycles in different types of primitive gilt. Journal of Reproduction and Fertility, 73, 159–164Google Scholar
  18. hart, I.C. and Linzell, J.L., 1977. An analysis of specific stimuli causing the release of prolactin and growth hormone at milking in the goat. Journal of Endocrinology, 72, 163–171Google Scholar
  19. Holst, J.J., Grönholt, R. and Schaffalitzky de Muckadell, O.B., 1981. Nervous control of pancreatic endocrine secretion in pigs. Insulin and glucagon responses to electrical stimulation of the vagus nerves. Acta Physiologica Scandinavica, 111, 1–7Google Scholar
  20. Inoue, K., Kawano, T., Shima, K., Kim, T., Suzuki, T., Tobe, T., Takeyama, M. and Yaijma, H., 1983. Effect of synthetic chicken vasoactive intestinal peptide on pancreatic blood flow and on exocrine and endocrine secretions of the pancreas in dogs. Digestive Diseases and Sciences, 28, 724–732Google Scholar
  21. Kendall, J.Z., Richards, G.E. and Shih, L.N., 1983. Effect of haloperidol, suckling, oxytocin and hand milking on plasma relaxin and prolactin concentrations in cyclic and lactating pigs. Journal of Reproduction and Fertility, 69, 271–277Google Scholar
  22. Lankisch, P.G., 1980. Trophic effect of gastrointestinal hormones. In: Clinics in Gastroenterology Hormones, Vol. 9, No. 3, (W.B. Saunders)Google Scholar
  23. Larsson, L.T., Fahrenkrug, J., Schaffalitzky de Muckadell, O.B., Sundler, F., Håkanson, R. and Rehfeld, J.F., 1976. Localization of vasoactive intestinal polypeptide (VIP) to central and peripheral neurons. Proceedings of the National Academy of Sciences of the United States of America, 73, 3197–3200Google Scholar
  24. Lindén, A., Eriksson, M., Carlquist, M. and Uvnäs-Moberg, K., 1987. Plasma levels of gastrin, somatostatin, and cholecystokinin immunoreactivity during pregnancy and lactation in dogs. Gastroenterology, 92, 578–584Google Scholar
  25. Lundberg, J.M., Änggård, A., Fahrenkrug, J., Hökfelt, T. and Mutt, V., 1980. Vasoactive intestinal polypeptide in cholinergic neurons of exocrine glands. Possible functional significance of coexisting transmittors for vasodilatation and secretion. Proceedings of the National Academy of Sciences of the United States of America, 77, 1651–1656Google Scholar
  26. Mattioli, M. and Seren, E., 1985. Effects of bromocriptine treatment during lactational anestrus in pigs. In: F.Ellendorff and F.Elsaesser (eds), Endocrine Causes of Seasonal and Lactational Anestrus in Farm Animals, (Martinus Nijhoff, Boston), 165–178Google Scholar
  27. Mattioli, M., De Rensis, F., Galeati, G. and Seren, E., 1986. Endocrine changes during lactation at weaning and after weaning in the sow. International Pig Veterinary Society Congress Proceedings, 35Google Scholar
  28. Mulloy, A.L. and Malven, P.V., 1979. Relationship between concentrations of porcine prolactin in blood serum and milk of lactating sows. Journal of Animal Science, 48, 876–881Google Scholar
  29. Nilsson, G., 1975. Increased plasma gastrin levels in connection with inhibition of gastric acid responses to sham feeding following bulbar perfusion with acid in dogs. Scandinavian Journal of Gastroenterology, 10, 273–277Google Scholar
  30. Rodriguez, H. and Kunavongkrit, A., 1983. Chronical venous catheterization for frequent blood sampling in unrestrained pigs. Acta Veterinaria Scandinavica, 24, 318–320Google Scholar
  31. Said, S.I., 1982. Vasoactive intestinal peptide. Advances in Peptide Hormone Research Series, (Raven Press, New York)Google Scholar
  32. SAS Institute Inc., 1985. SAS User's guide: Statistics, Version 5 Edition, (SAS Institute Inc., Cary, NC)Google Scholar
  33. Schmidt-Polex, B., Schams, D. and Karg, H., 1976. Prolaktin-neben Oxytocin ein Indikatorhormon für neuroendokrine Vorgänge bei der Stimulation der Milchdrüse. Deutsche Molkerei-Zeitung, 97, 707–708Google Scholar
  34. Stock, S., Uvnäs-Moberg, K., 1987. L-vasopressin inhibits oxytocin induced increases of plasma levels of insulin in conscious dogs. Acta Physiologica Scandinavica, 130, 55–61Google Scholar
  35. Stock, S., Uvnäs-Moberg, K., 1989. Interaction between gastrin-17 and oxytocin on plasma levels of insulin, glucagon and glucose in conscious dogs. Acta Physiologica Scandinavica, 135, 559–564Google Scholar
  36. Tucker, H.A., 1974. Endocrinological control of lactation. In: B.L.Larson and V.R.Smith (eds), Lactation, (Academic Press, New York)Google Scholar
  37. Uvnäs-Moberg, K., 1983. Release of gastrointestinal peptides in response to vagal activation induced by electrical stimulation, feeding and suckling. Journal of the Autonomic Nervous System, 9, 141–155Google Scholar
  38. Uvnäs-Moberg, K., 1987. Gastrointestinal hormones and pathophysiology of functional gastrointestinal disorder. Scandinavian Journal of Gastroenterology, 22, (suppl. 128), 138–146Google Scholar
  39. Uvnäs-Moberg, K. and Eriksson, M., 1983. Release of gastrin and insulin in response to suckling in lactating dogs. Acta Physiologica Scandinavica, 119, 181–185Google Scholar
  40. Uvnäs-Moberg, K., Eriksson, M., Blomquist, L.-E., Kunavongkrit, A. and Einarsson, S., 1984. Influence of suckling and feeding on insulin, gastrin, somatostatin and VIP levels in peripheral venous blood of lactating sows. Acta Physiologica Scandinavica, 121, 31–38Google Scholar
  41. Uvnäs-Wallensten, K., 1976. Gastrin release and HCl secretion induced by electrical vagal stimulation in the cat. Acta Physiologica Scandinvavica, Suppl. 438Google Scholar
  42. Uvnäs-Wallensten, K. and Nilsson, G., 1977. Insulin release to vagal stimulation in anesthetized cats. Hormone and Metabolic Research, 9, 175–181Google Scholar
  43. vanLandeghem, A.A.J. and van deWiel, D.F.M., 1978. Radioimmunoassay for porcine prolactin plasma levels during lactation, suckling and weaning and after TRH administration. Acta Endocrinologica, 88, 653–667Google Scholar
  44. Welch, A.R. and Bacter, M.R., 1986. Responses of newborn piglets to thermal and tactile properties of their environment. Applied Animal Behaviour Science, 15, 203–215Google Scholar
  45. Whitacre, M.D. and Threlfall, W.R., 1981. Effects of ergocryptine on plasma prolactin, luteinizing hormone and progesterone in the periparturient sow. American Journal of Veterinary Research, 42, 1538–1541Google Scholar
  46. Widström, A.-M., Winberg, J., Werner, S., Svensson, K., Posloncec, B. and Uvnäs-Moberg, K., 1988. Breast feeding-induced effects on plasma gastrin and somatostatin levels and their correlation with milk yield in lactating females. Early Human Development, 16, 293–301Google Scholar

Copyright information

© Kluwer Academic Publishers bv 1991

Authors and Affiliations

  • B. Algers
    • 1
  • A. Madej
    • 2
  • S. Rojanasthien
    • 3
  • K. Uvnäs-Moberg
    • 4
  1. 1.Department of Animal HygieneSwedish University of Agricultural ScienceStockholmSweden
  2. 2.Department of Clinical ChemistrySwedish University of Agricultural ScienceStockholmSweden
  3. 3.Department of Obstetrics and GynaecologySwedish University of Agricultural ScienceStockholmSweden
  4. 4.Department of PharmacologyKarolinska InstituteStockholmSweden

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