Current Genetics

, Volume 3, Issue 2, pp 151–156 | Cite as

Plasmid-like DNA is part of mitochondrial DNA in Podospora anserina

  • Ulrich Kück
  • Ulf Stahl
  • Karl Esser
Article

Summary

As previously reported, a ccc DNA with a contour length of 0.75 µm and molecular weight of 2.4 kb (termed plasmid-like, p1DNA) is the causative agent of senescence in the fungus Podospora anserina. Its postulated location in mtDNA was proved correct by the following experiments:
  1. 1.

    Restriction analysis of mtDNA resulted in different molecular weights for both, juvenile (95 kb) and senescent (30 kb) mtDNA. The construction of a detailed restriction map made evident the fact that senescent mtDNA comprises only a part of its juvenile counterpart.

     
  2. 2.

    Hybridization experiments (Southern blots) between 3H-labelled plDNA and mtDNA cleaved by restriction juvenile mtDNA are homologous to plDNA.

     
  3. 3.

    Fine mapping experiments (construction of restriction maps and heteroduplex experiments) between plDNA integrated into a bacterial vector and its postulated equivalent, derived from juvenile mtDNA and also integrated into a bacterial vector, allowed a precise determination of the site of plDNA insertion within the juvenile mtDNA.

     

All of these data fit into a previously published model in which, during aging, plDNA is excised from mtDNA and becomes autonomous for replication and function.

Key words

Podospora anserina plDNA Integral part of mtDNA 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Ulrich Kück
    • 1
  • Ulf Stahl
    • 1
  • Karl Esser
    • 1
  1. 1.Lehrstuhl für Allgemeine BotanikRuhr-UniversitätBochum 1Federal Republic of Germany

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