Current Genetics

, Volume 5, Issue 2, pp 143–147 | Cite as

Genetic map of mitochondrial DNA in Podospora anserina

  • Ulrich Kück
  • Karl Esser
Original Articles


In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (functional) map, 70% of the chondriome was cloned in E. coli vectors. Using mitochondrial genes from Saccharomyces cerevisiae, six structural genes were located on the Podospora chondriome by cross hybridization experiments. There is strong evidence that the plasmid is inserted into the cytochrome b gene. A comparison of the genetic map of the Podospora chondriome with those of Neurospora crassa and Aspergillus nidulans exhibits a rather good accordance with respect to the sequence of genes.

Key words

Podospora anserina Mitochondrial DNA cloning Restriction and gene mapping 


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  1. Agsteribbe E, Samallo J, de Vries H, Hensgens LAM, Grivell LA (1980) In: Kroon AM, Saccone C (eds) The Organization and Expression of the Mitochondrial Genome. Elsevier/North Holland, Amsterdam, pp 51–60Google Scholar
  2. Chang ACY, Cohen SN (1978) J Bacteriol 134:1141–1156Google Scholar
  3. Fox TD (1979) J Mol Biol 130:63–82Google Scholar
  4. Fox TD, Leaver CJ (1981) Cell 26:315–323Google Scholar
  5. Haid A, Grosch G, Schmelzer C, Schweyen RJ, Kaudewitz F (1980) Curr Genet 1:155–161Google Scholar
  6. Kück U, Stahl U, Esser K (1981) Curr Genet 3:151–156Google Scholar
  7. Kück U, Stahl U, Lhermitte A, Esser K (1980) Curr Genet 2:97–101Google Scholar
  8. Lazarus CM, Küntzel H (1981) Curr Genet 4:99–107Google Scholar
  9. Lazarus CM, Lünsdorf H, Hahn U, Stepien PP, Küntzel H (1980) Mol Gen Genet 177:389–397Google Scholar
  10. Macino G (1980) J Biol Chem 255:10563–10565Google Scholar
  11. Macino G, Scazzocchio C, Waring RB, McPhail Berks M, Wayne Davies R (1980) Nature 288:404–406Google Scholar
  12. Mannella CA, Goewert RR, Lambowitz AM (1979) Cell 18:1197–1207Google Scholar
  13. Schweyen RJ, Kaudewitz F (1976) Mol Gen Genet 149:311–322Google Scholar
  14. Sebald W (1977) Biochim Biophys Acta 463:1–27Google Scholar
  15. Southern E (1979) In: Colowick SP, Kaplan NO (eds) Methods in Enzymology, vol 68. Academic Press, New York, pp 152–176Google Scholar
  16. Stahl U, Kück U, Tudzynski P, Esser K (1980) Mol Gen Genet 178:639–646Google Scholar
  17. Stahl U, Lemke PA, Tudzynski P, Kück U, Esser K (1978) Mol Gen Genet 162:341–343Google Scholar
  18. Stahl U, Tudzynski P, Kück U, Esser K (1982) Proc Natl Acad Sci USA (in press)Google Scholar
  19. Wesolowski M, Algeri A, Fukuhara H (1981) Curr Genet 3:157–162Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Ulrich Kück
    • 1
  • Karl Esser
    • 1
  1. 1.Lehrstuhl für Allgemeine Botanik, Ruhr-UniversitätBochumGermany

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