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Current Genetics

, Volume 5, Issue 2, pp 143–147 | Cite as

Genetic map of mitochondrial DNA in Podospora anserina

  • Ulrich Kück
  • Karl Esser
Original Articles

Summary

In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (functional) map, 70% of the chondriome was cloned in E. coli vectors. Using mitochondrial genes from Saccharomyces cerevisiae, six structural genes were located on the Podospora chondriome by cross hybridization experiments. There is strong evidence that the plasmid is inserted into the cytochrome b gene. A comparison of the genetic map of the Podospora chondriome with those of Neurospora crassa and Aspergillus nidulans exhibits a rather good accordance with respect to the sequence of genes.

Key words

Podospora anserina Mitochondrial DNA cloning Restriction and gene mapping 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Ulrich Kück
    • 1
  • Karl Esser
    • 1
  1. 1.Lehrstuhl für Allgemeine Botanik, Ruhr-UniversitätBochumGermany

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