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Current Genetics

, Volume 7, Issue 1, pp 7–12 | Cite as

Molecular cloning and characterization of the chloroplast ribosomal RNA genes from Spirodela oligorhiza

  • Ronald J. A. Keus
  • Dick J. Roovers
  • Harm van Heerikhuizen
  • Gert S. P. Groot
Article

Summary

The organization of the chloroplast ribosomal RNA genes in Spirodela oligorhiza has been determined. We have therefore characterized two cloned BamHI fragments containing the genes for the large and small rRNA genes, and two PstI fragments, each containing one of the two large, invertedly repeated regions of chloroplast DNA. Characterization was performed by restriction endonuclease mapping, DNA/RNA hybridization and electronmicroscopic R-loop analysis.

The results show that the rRNA genes are present in the order 16S, 23S, 4.5S and 5S. The 16S (1.5 kb) and 23S (2.9 kb) rRNA genes are separated by a spacer of 2.25 kb. There appears to be a close resemblance between the organization of the chloroplast rRNA genes in Spirodela oligorhiza and other higher plants so far examined.

Key words

Chloroplast DNA Spirodela oligorhiza Ribosomal RNA genes Physical map 

Abbreviations

cp

chloroplast

rRNA

ribosomal RNA

(k)bp

(kilo) base pairs

TPNS

tri-isopropyl naphtalene sulfonic acid

DTT

dithiothreitol

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Ronald J. A. Keus
    • 1
  • Dick J. Roovers
    • 1
  • Harm van Heerikhuizen
    • 1
  • Gert S. P. Groot
    • 1
  1. 1.Biochemical LaboratoryFree UniversityHV AmsterdamThe Netherlands

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