Current Genetics

, Volume 13, Issue 5, pp 425–432 | Cite as

Both α-tubulin genes are transcriptionally active in Stylonchyia lemnae

  • Elke Helftenbein
  • Elke Müller
Original Articles
Received:

Summary

Macronuclear DNA of the hypotrichous ciliate Stylonychia lemnae contains two size-classes of molecules coding for α-tubulin. Analysis of their coding regions demonstrates that these two size-classes represent two different functional α-tubulin genes, α1 and α2; a comparison of these regions shows a 97% homology in their nucleotide sequence and 98.5% in their amino acid sequence. S1-mapping experiments indicate that both α1-and α2-tubulin genes are transcribed. The 5′ and 3′ noncoding regions flanking the α1- and α2-tubulin genes differ in both length and nucleotide sequence within one strain. When different strains are compared, however, identical non-coding regions and slightly varying coding regions can be found in DNA molecules containing the α1-tubulin genes.

Key words

Stylonychia α-tubulin genes Transcription 
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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Elke Helftenbein
    • 1
  • Elke Müller
    • 1
  1. 1.Universität Tübingen, Biologie III7400TübingenGermany

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