, Volume 104, Issue 1, pp 1–11 | Cite as

Host plant influence on chemical defense in conifer sawflies (Hymenoptera: Diprionidae)

  • S. G. CodellaJr.
  • K. F. Raffa
Original Paper


Host diet affects the defensive efficacy of Neodiprion sawflies. In laboratory assays with wood ants (Formica obscuripes), secretions from larvae reared on Pinus banksiana were the most repellent, while those from P. resinosa feeders were the least so. This was explained diterpene resin acid content, but not total monoterpene content. The terpene content of regurgitant generally reflected dietary concentrations. Compounds were sequestered nonselectively by larvae. Host-based differences in defense persisted at the behavioral level. P. banksiana feeders regurgitated greater volumes of fluid and were less likely to be disabled or killed by ants in one-on-one interactions than were larvae fed on P. resinosa. The defensive advantages of host diet conflicted with developmental requirements. N. sertifer reared on P. banksiana (the best diet for defense in all cases) had lower cocoon weights (a correlate of fecundity) than those reared on other diets, and had prolonged larval development compared to insects fed P. sylvestris. No such tradeoff was detected in N. lecontei. Larvae of both species strongly preferred P. banksiana over P. resinosa in feeding choice assays.

Key words

Antipredator Tradeoffs Neodiprion Formica Pinus 


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  1. Aldrich Chemical Company (1985) Diazald (Technical information bulletin AL-113). Aldrich Chemical Company, MilwaukeeGoogle Scholar
  2. Aldrich JR, Blum MS, Lloyd HA, Fales HM (1978) Pentatomid natural products. chemistry and morphology of the III–IV dorsal abdominal glands of adults. J Chem Ecol 4:161–172Google Scholar
  3. Belofsky G, Bowers MD, Janzen S, Stermitz F (1989) Iridoid glycosides of Aureolaria flava and their sequestration by Euphydryas phaeton butterflies. Phytochemistry 28:1601–1604Google Scholar
  4. Benjamin DM (1955) The biology and ecology of the redheaded pine sawfly (Technical bulletin 1118). United States Department of Agriculture. Washington, DCGoogle Scholar
  5. Bernays EA (1989) Host range in phytophagous insects: the potential role of generalist predators. Evol Ecol 3:299–311Google Scholar
  6. Bjorkman C, Larsson S (1991) Pine sawfly defence and variation in host plant resin acids: a trade-off with growth. Ecol Entomol 16:283–290Google Scholar
  7. Bowers MD (1990) Recycling plant natural products for defense. In: Evans DL, Schmidt JO (eds) Insect defenses: adaptive mechanisms and strategies of prey and predators. State University of New York Press, Albany, pp 353–386Google Scholar
  8. Brower LP, MacEvoy PB, Williamson KL, Flannery MA (1972) Variation in cardiac glycoside content of monarch butterflies from natural populations in eastern North America. Science 177:426–429Google Scholar
  9. Brower LP, Seiber JN, Nelson CJ, Lynch SP, Tuskes PM (1982) Plant-determined variation in the cardenolide content, thinlayer chromatography profiles, and emetic potency of monarch butterflies, Danaus plexippus, reared on the milkweed, Asclepias eriocarpa, in California. J Chem Ecol 8:579–629Google Scholar
  10. Brower LP, Seiber JN, Nelson CJ, Lynch SP, Holland MM (1984a) Plant-determined variation in the cardenolide content, thinlayer chromatography profiles, and emetic potency of monarch butterflies, Danaus plexippus L., reared on milkweed plants in California. 2. Asclepias speciosa. J Chem Ecol 10:601–633Google Scholar
  11. Brower LP, Seiber JN, Nelson CJ, Lynch SP, Hoggard MP, Cohen JA (1984b) Plant-determined variation in cardenolide content and thin-layer chromatography profiles of monarch butterflies, Danaus plexippus, reared on milkweed plants in California. 2. Asclepias californica. J Chem Ecol 10:1823–1857Google Scholar
  12. Buratti L, Allais JP, Barbier M (1988) The role of resin acids in the relationship between Scots pine and the sawfly, Diprion pini (Hymenoptera: Diprionidae). I. Resin acids in the needles. In: Mattson WJ, Levieux J, Bernard-Dagan C (eds) Mechanisms of woody plant defenses against insects: search for pattern. Springer, Berlin Heidelberg New York, pp 171–187Google Scholar
  13. Codella SG (1994) Ecology and evolution of defense strategies in conifer sawflies, with particular reference to wood ant predation (Hymenoptera: Diprionidae, Formicidae). PhD thesis, University of Wisconsin, MadisonGoogle Scholar
  14. Codella SG, Raffa KF (1993) Defense strategies of folivorous sawflies. In: Wagner M, Raffa KF (eds) Sawfly life history adaptations to woody plants. Academic Press, San Diego, pp 261–294Google Scholar
  15. Codella SG, Raffa KF (in press a) Contributions of female oviposition patterns and larval behavior to group defense in conifer sawflies (Hymenoptera: Diprionidae). OecologiaGoogle Scholar
  16. Codella SG, Raffa KF (in press b) Individual and social components of wood ant response to conifer sawfly defense behaviour (Hymenoptera: Formicidae, Diprionidae). Anim BehavGoogle Scholar
  17. Codella SG, Fogal WH, Raffa KF (1991) The effect of host variability on growth and performance of the introduced pine sawfly, Diprion similis. Can J For Res 21:1668–1674Google Scholar
  18. Cohen JA (1985) Differences and similarities in cardenolide contents of queen and monarch butterflies in Florida and their ecological and evolutionary implications. J Chem Ecol 11:85–103Google Scholar
  19. Common IFB, Bellas TE (1977) Regurgitation of host-plant oil from a foregut diverticulum in the larvae of Myrascia megalocentra and M. bracteatella (Lepidoptera: Oecophoridae). J Aust Entomol Soc 16:141–147Google Scholar
  20. Coppel HC, Benjamin DM (1965) Bionomics of the nearctic pinefeeding diprionids. Annu Rev Entomol 10:69–96Google Scholar
  21. Cosens D, Toussaint N (1986) The dynamic nature of the activities of the wood ant Formica aquilonia foraging to static food resources within a laboratory habitat. Physiol Entomol 11: 383–395Google Scholar
  22. Duffey SS (1980) Sequestration of plant natural products by insects. Annu Rev Entomol 25:447–477Google Scholar
  23. Duffey SS, Scudder GGE (1974) Cardiac glycosides in Oncopeltus fasciatus (Dallas) (Hemiptera: Lygaeidae). I. The uptake and distribution of natural cardenolides in the body. Can J Zool 52:283–290Google Scholar
  24. Eisner T, Johnessee JS, Carrel J, Hendry LB, Meinwald J (1974) Defensive use by an insect of a plant resin. Science 184: 996–999Google Scholar
  25. Entwistle PF, Adams PHW, Evans HF (1983) Employing defensive regurgitation to estimate levels of nuclear polyhedrosis virus infection in Gilpinia hercyniae (Hymenoptera: Diprionidae) larvae. J Invert Pathol 41:262–264Google Scholar
  26. Foster DO, Zinkel DF (1982) Qualitative and quantitative analysis of diterpene resin acids by glass capillary gas-liquid chromatography. J Chromatogr 248:89–98Google Scholar
  27. Gardner DR, Stermitz FR (1988) Host plant utilization and iridoid glycoside sequestration by Euphydryas ancia (Lepidoptera: Nymphalidae). J Chem Ecol 12:2147–2168Google Scholar
  28. Geri C, Allais J-P, Auger M-A (1993) Effects of plant chemistry and phenology on sawfly behavior and development. In: Wagner M, Raffa KF (eds) Sawfly life history adaptations to woody plants. Academic Press, San Diego, pp 173–210Google Scholar
  29. Gosswald K (1989) Die Waldameise Band I. Biologische Grundlagen, Ökologie und Verhalten, AULA, WiesbadenGoogle Scholar
  30. Haack RA, Mattson WJ (1993) Life history patterns of North American tree-feeding sawflies. In: Wagner M, Raffa KF (eds) Sawfly life history adaptations to woody plants. San Diego, Academic Press, pp 503–545Google Scholar
  31. Herbers JM (1979) Caste-based polyethism in a mound-building ant species. Am Midl Nat 101:69–75Google Scholar
  32. Holloway GJ, Jong PW de, Brakefield PM, Vos H de (1991) Chemical defence in ladybird beetles (Coccinellidae). I. Distribution of coccinelline and individual variation in defence in 7-spot ladybirds. Chemoecology 2:7–14Google Scholar
  33. Honda K (1983) Defensive potential of components of the larval osmeterial secretion of papilionid butterflies against ants. Physiol Entomol 8:173–179Google Scholar
  34. Ikeda T, Matsumura F, Benjamin DM (1977) Chemical basis for feeding adaptation of pine sawflies Neodiprion rugifrons and Neodiprion swainei. Science 197:497–499Google Scholar
  35. Jones CG, Hess TA, Whitman DW, Silk PJ, Blum MS (1986) Idiosyncratic variation in chemical defenses among individual generalist grasshoppers. J Chem Ecol 12:749–761Google Scholar
  36. Kapler JE, Benjamin DM (1960) The biology and ecology of the red pine sawfly in Wisconsin. For Sci 6:253–268Google Scholar
  37. Knerer G (1984) Diprionid sawflies: biological topics and rearing techniques. Bull Entomol Soc Am 30:53–57Google Scholar
  38. Knerer G (1990) Neodiprion maurus Rohwer (Hymenoptera: Symphyta), a rare northern sawfly with a unique life history. Zool Anz 225:37–44Google Scholar
  39. Knerer G, Atwood CE (1973) Diprionid sawflies: polymorphism and speciation. Science 179:1090–1099Google Scholar
  40. Kolomeits NG, Stadnitskii GV, Vorontzov AI (1972) The European pine sawfly: distribution, biology, economic importance, natural enemies and control. USDA Forest Service and National Science Foundation, WashingtonGoogle Scholar
  41. Kossuth SV, Muse HD (1986) Cortical monoterpene variation among slash pine ramets by season, aspect, crown position, and bud vigor. For Sci 32:605–613Google Scholar
  42. Larsson S, Bjorkman C, Gref R (1986) Responses of Neodiprion sertifer (Hym., Diprionidae) larvae to variation in needle resin acid concentration in Scots pine. Oecologia 70:77–84Google Scholar
  43. Larsson S, Bjorkman C, Kidd NAC (1993) Outbreaks in diprionid sawflies: why some species and not others? In: Wagner M, Raffa KF (eds) Sawfly life history adaptations to woody plants. Academic Press, San Diego, pp 453–484Google Scholar
  44. Lyons LA (1964) The European pine sawfly, Neodiprion sertifer (Geoff.) (Hymenoptera: Diprionidae). a review with emphasis on studies in Ontario. Proc Entomol Soc Ontario 94:5–37Google Scholar
  45. Marsh N, Rothschild M (1974) Aposematic and cryptic Lepidoptera tested on the mouse. J Zool 174:89–122Google Scholar
  46. McKey D (1979) The distribution of secondary compounds within plants. In: Rosenthal GA, Janzen DH (eds) Herbivores: their interaction with secondary plant metabolites. Academic Press, New York, pp 55–133Google Scholar
  47. McNeil JN, Delisle J, Finnegan RJ (1978) Seasonal predatory activity of the introduced red wood ant, Formica lugubris (Hymenoptera: Formicidae) at Valcartier, Quebec, in 1976. Can Entomol 110:85–90Google Scholar
  48. Moore PP, Hanover JW (1987) Variation in yield of blue spruce monoterpenes associated with crown position and frequency of resin canals. For Sci 33:1081–1088Google Scholar
  49. Oiofsson E (1992) Predation by Formica polyctena Forster (Hym., Formicidae) on newly emerged larvae of Neodiprion sertifer (Geoffroy) (Hym., Diprionidae). J Appl Entomol 114:315–319Google Scholar
  50. Pasteels JM, Gregoire J-C, Rowell-Rahier M (1983) The chemical ecology of defense in arthropods. Annu Rev Entomol 28: 263–289Google Scholar
  51. Prop N (1960) Protection against brids and parasites in some species of tenthredinid larvae. Arch Neerl Zool 13:380–447Google Scholar
  52. Raffa KF, Steffeck RJ (1988) Computation of response factors for quantitative analysis of monoterpenes by gas-liquid chromatography. J Chem Ecol 14:1385–1389Google Scholar
  53. Ross HH (1955) The taxonomy and evolution of the sawfly genus Neodiprion. For Sci 1:196–209Google Scholar
  54. Rudloff E von (1975) Volatile leaf oil analysis in chemosystematic studies of North American conifers. Biochem Syst Ecol 2: 131–167Google Scholar
  55. Rudloff E von, Lapp MS, McGinn RG (1985) Chemosystematic studies in the genus Pinus. V. Variation in the leaf oil terpene composition of young and old lodgepole pine trees from different moisture regimes near Prince George, British Columbia. Can J For Res 15:801–808Google Scholar
  56. SAS (1985) SAS user's guide: statistics. SAS Institute, CaryGoogle Scholar
  57. Schuh BA, Benjamin DM (1984) The chemical feeding ecology of Neodiprion dubiosis Schedl, N. rugifrons Midd., and N. lecontei (Fitch) on jack pine (Pinus banksiana Lamb.). J Chem Ecol 10:1071–1079Google Scholar
  58. Siegel S, Castellan NJ (1988) Nonparametric statistics for the behavioral sciences, 2nd edn. McGraw-Hill, New YorkGoogle Scholar
  59. Smirnoff WA (1960) Observations on the migration of larvae of Neodiprion swainei Midd. (Hymenoptera: Tenthredinidae). Can Entomol 92:957–958Google Scholar
  60. Stradling DJ (1987) Nutritional ecology of ants. In: Slansky F, Rodriguez JG (eds) Nutritional ecology of insects, mites, spiders and related invertebrates. Wiley, New York, pp 927–969Google Scholar
  61. Teras I (1982) The climbing activity of larvae of the European pine sawfly, Neodiprion sertifer (Hymenoptera: Diprionidae), knocked down from small pines. Notul Entomol 62:9–12Google Scholar
  62. Tripp HA (1960) Spathimeigenia spinigera Townsend (Diptera: Tachinidae), a parasite of Neodiprion swainei Midd. (Hymenoptera: Tenthredinidae). Can Entomol 92:347–359Google Scholar
  63. Whitman DW (1990) Allomones: chemicals for defense. In: Evans DL, Schmidt JO (eds) Insect defenses: adaptive mechanisms and strategies of prey and predators. State University of New York Press, Albany, pp 289–351Google Scholar
  64. Zar JH (1984) Biostatistical analysis, 2nd edn. Prentice-Hall, Englewood CliffsGoogle Scholar
  65. Zavarin E, Cobbs FW Jr, Bergot J, Barber HW (1971) Variation of the Pinus ponderosa needle oil with season and needle age. Phytochemistry 10:3107–3114Google Scholar
  66. Zinkel DF, Han JS (1986) GLC determination of the resin acid composition in rosins and oleoresins: state of the art. Naval Stores Rev 96:14–19Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. G. CodellaJr.
    • 1
  • K. F. Raffa
    • 2
  1. 1.Department of EntomologyUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Department of BiologyNorthland CollegeAshlandUSA

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