Epidermal growth factor and transforming growth factor alpha regulate extracellular matrix production by embryonic mouse palatal mesenchymal cells cultured on a variety of substrata
- 37 Downloads
Mouse embryonic palatal mesenchymal (MEPM) cells were cultured either on plastic tissue culture dishes or on the surface of three-dimensional collagen gels or within collagen gel matrices in DMEM/F12 medium containing 2.5% donor calf serum. MEPM cells proliferated exponentially when cultured on collagen or on plastic. Cells cultured within collagen gels did not proliferate but remained viable. Addition of 10 ng/ml epidermal growth factor (EGF) or transforming growth factor alpha (TGFα) stimulated the proliferation of those cells cultured on plastic or on collagen but not those cultured within collagen gels. Immunocytochemical analysis revealed that MEPM cells synthesise collagen types I, III, IV, V, VI and IX; fibronectin, heparan sulphate proteoglycan, laminin and tenascin in vitro. These molecules are all present in the developing palate in vivo. EGF and TGFα produced a generalised stimulation of extracellular matrix (ECM) synthesis by MEPM cells in vitro. Biochemical analysis indicated that cells cultured within collagen gels had the highest intrinsic rate of protein synthesis. On all substrata neither EGF nor TGFα markedly altered the types of ECM molecules synthesised but rather caused a general increase in the total amount produced. This stimulation was most marked where the cells were cultured within collagen gels. The lack of stimulation of proliferation of MEPM cells cultured within collagen gels (i.e. in a physiologically-relevant environment) by EGF or TGFα together with the marked stimulation of ECM synthesis suggests that these factors may act as differentiation signals via their effects on ECM production.
Key wordsPalate development Epidermal growth factor Transforming growth factor alpha Extracellular matrix
Unable to display preview. Download preview PDF.
- Bissell MJ, Hall HG, Parry G (1982) How does the extracellular matrix direct gene expression? J Theor Biol 99:36–81Google Scholar
- Ferguson MWJ, Honig LS (1984) Epithelial-mesenchymal interactions during vertebrate palatogenesis. In: Zimmerman EF (ed) Current topics in developmental biology, vol. 19. Palate development: normal and abnormal, cellular and molecular aspects. Academic Press, New York, pp 137–164Google Scholar
- Ingber DE, Madri JA, Folkman J (1987) Endothelial growth factors and extracellular matrix regulate DNA synthesis through modulation of cell and nuclear expansion. In Vitro 23:387–394Google Scholar
- Juva K, Prockop DJ (1966) Modified procedure for the assay of H3- or C14-labelled hydroxyproline. Ann Biochem 15:77–83Google Scholar
- Morgan KS, Pratt RM (1985) Fibronectin and laminin expression in human and mouse embryonic craniofacial tissues. J Cell Biol 101:378Google Scholar
- Pratt RM, Kim CS, Grove RI (1984) The role of glucocorticoids and epidermal growth factor in normal and abnormal palate development. In: Zimmermann EF (ed) Current topics in developmental biology, vol. 19. Palate development: normal and abnormal, cellular and molecular aspects. Academic Press, New York, pp 81–102Google Scholar
- Sharpe PM, Ferguson MWJ (1988) Mesenchymal influences on epithelial differentiation in developing systems. J Cell Sci [Suppl] 10:195–230Google Scholar
- Theiler K (1972) The House Mouse. Development and Normal Stages from Fertilization to Four Weeks of Age. Springer, Berlin Heidelberg New YorkGoogle Scholar
- Yoneda T, Pratt RM (1981) Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor. Science 213:63–565Google Scholar