Advertisement

The fine structure and development of the seminal vesicles and prostate in the fetal rat

  • Charles J. Flickinger
Article

Summary

The development of the seminal vesicle from the Wolffian duct and of the prostate from the urogenital sinus has been studied in rat fetuses from day 14 of gestation to birth with the use of the electron microscope. Prior to the onset of androgen secretion, the cells of the urogenital sinus and the caudal part of the Wolffian duct have a simple undifferentiated appearance. After the onset of androgen secretion by the fetal testes at day 15, “intracytoplasmic confronting cisternae” of the granular reticulum appear in both urogenital sinus and Wolffian duct. Portions of the granular endoplasmic reticulum of the urogenital sinus become distended with a finely granular, moderately dense material. In the urogenital sinus, many hemidesmosomes are formed at the basal surface of the epithelium. Specializations of the extracellular materials are present opposite the hemidesmosomes. The formation of the seminal vesicles and the prostate begins at day 18–19 of gestation. The cells of the seminal vesicle are taller than the Wolffian duct cells from which they arise, the granular endoplasmic reticulum increases moderately in amount, and a patent lumen is formed. The cells of the fetal prostate do not differ greatly from those of the urogenital sinus from which they arise except that the prostatic cells initially lack hemidesmosomes. The fine structural changes are discussed in relation to the onset of fetal androgen secretion, the formation of the organs, and the functions of the cells in adult life.

Key-Words

Seminal vesicle Prostate Fetal rat Ultrastructure Development 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allison, V. F.: Ultrastructural changes in the seminal vesicle epithelium of the rat following castration and androgen. Anat. Rec. 148, 254 (1964) (Abstr.).Google Scholar
  2. Belt, W. D., Cavazos, L. F.: Fine structure alterations of the epithelium of the hamster seminal vesicle following treatment with diethylstilbestrol. Anat. Rec. 157, 212 (1967) (Abstr.).Google Scholar
  3. Brandes, D.: The fine structure and histochemistry of prostatic glands in relation to sex hormones. Int. Rev. Cytol. 20, 207–276 (1966).Google Scholar
  4. —, Groth, D. P.: The fine structure of the rat prostatic complex. Exp. Cell Res. 23, 159–175 (1961).Google Scholar
  5. Brewer, N. L.: Sex differentiation of the fetal mouse in vitro. Ph. D. Thesis, University of Chicago (1962).Google Scholar
  6. Cavazos, L. F., Belt, W. D.: Fine structure of the seminal vesicle of the hamster during development. Anat. Rec. 151, 333 (1965) (Abstr.).Google Scholar
  7. Chang, J. P., Gibley, C. W.: Ultrastructure of tumor cells during mitosis. Cancer Res. 28, 521–534 (1968).Google Scholar
  8. Deane, H. W.: Electron microscopic observations on the mouse seminal vesicle. Nat. Cancer Inst. Monogr. 12, 63–83 (1963).Google Scholar
  9. —, Wurzelmann, S.: Electron microscopic observations on the postnatal differentiation of the seminal vesicle epithelium of the laboratory mouse. Amer. J. Anat. 117, 91–134 (1965).Google Scholar
  10. Douglas, W. H. J., Ripley, R. C., Ellis, R. A.: Enzymatic digestion of desmosome and hemidesmosome plaques performed on ultrathin sections. J. Cell Biol. 44, 211–215 (1970).Google Scholar
  11. Epstein, M. A.: Some unusual features of fine structure observed in HeLa cells. J. biophys. biochem. Cytol. 10, 153–162 (1961).Google Scholar
  12. Flickinger, C. J.: The postnatal development of the Sertoli cells of the mouse. Z. Zellforsch. 78, 92–113 (1967).Google Scholar
  13. —: The fine structure of the Wolffian duct and cytodifferentiation of the epididymis in fetal rats. Z. Zellforsch. 96, 344–360 (1969).Google Scholar
  14. - Extracellular specializations associated with hemidesmosomes in the fetal rat urogenital sinus. Anat. Rec., in press.Google Scholar
  15. Glücksmann, A.: Cell deaths in normal vertebrate ontogeny. Biol. Rev. 26, 59–86 (1951).Google Scholar
  16. Hruban, Z., Swift, H., Rechcigl, M.: Fine structure of transplantable hepatomas of the rat. J. nat. Cancer Inst. 35, 459–495 (1965).Google Scholar
  17. Ito, S., Karnovsky, M. J.: Formaldehyde-glutaraldehyde fixatives containing trinitro compounds. J. Cell Biol. 39, 168A (1968) (Abstr.).Google Scholar
  18. Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A (1965) (Abstr.).Google Scholar
  19. Kelly, D. E.: Fine structure of desmosomes, hemidesmosomes, and an adepidermal globular layer in developing newt epidermis. J. Cell Biol. 28, 51–72 (1966).Google Scholar
  20. Kumegawa, M., Cattoni, M., Rose, G. G.: Electron microscopy of oral cells in vitro. II. Sub-surface and intracytoplasmic confronting cisternae in strain KB cells. J. Cell Biol. 36, 443–452 (1968).Google Scholar
  21. Leak, L. V., Caulfield, J. B., Burke, J. F., McKhann, C. F.: Electron microscopic studies on human fibromyxosarcoma. Cancer Res. 27, 261–285 (1967).Google Scholar
  22. Murray, R. G., Murray, A. S., Pizzo, A.: The fine structure of mitosis in rat thymic lymphocytes. J. Cell Biol. 26, 601–619 (1965).Google Scholar
  23. Odland, G. F.: The fine structure of the interrelationship of cells in the human epidermis. J. biophys. biochem. Cytol. 4, 529–538 (1958).Google Scholar
  24. Porter, K. R.: The submicroscopic structure of protoplasm. Harvey Lect. 51, 175–228 (1957).Google Scholar
  25. Price, D.: Normal development of the prostate and seminal vesicles of the rat, with a study of experimental post-natal modifications. Amer. J. Anat. 60, 79–125 (1936).Google Scholar
  26. —, Ortiz, E.: The role of fetal androgen in sex differentiation in mammals. In: Organogenesis (ed. R. L. Dehaan and H. Ursprung), p. 629–652. New York: Holt, Rinehart & Winston 1965.Google Scholar
  27. —, Pannebecker, R.: Comparative responsiveness of homologous sex ducts and accessory glands of fetal rats in culture. Arch. Anat. micr. Morph. exp. 48, 223–244 (1959).Google Scholar
  28. —, Williams-Ashman, H. G.: The accessory reproductive glands of mammals. In: Sex and internal secretions (ed. W. C. Young), vol. 1, p. 366–448. Baltimore: Williams & Wilkins Co. 1961.Google Scholar
  29. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).Google Scholar
  30. Riva, A.: Fine structure of human seminal vesicle epithelium. J. Anat. (Lond.) 101, 615 (1967) (Abstr.).Google Scholar
  31. Snell, R. S.: The fate of epidermal desmosomes in mammalian skin. Z. Zellforsch. 66, 471–487 (1965).Google Scholar
  32. Toner, P. G., Baillie, A. H.: Biochemical, histochemical, and ultrastructural changes in the mouse seminal vesicle after castration. J. Anat. (Lond.) 100, 173–188 (1966).Google Scholar
  33. Weiss, P., Ferris, W.: Electronmicrograms of larval amphibian epidermis. Exp. Cell Res. 6, 546–549 (1954).Google Scholar
  34. Wrobel, K.-H.: Morphological studies on the active secretory epithelium of the porcine seminal vesicle. Anat. Rec. 160, 454 (1968) (Abstr.).Google Scholar
  35. Zelickson, A. S.: Electron microscopy of skin and mucous membrane, p. 50–52. Springfield, Ill.: Ch. C. Thomas 1963.Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • Charles J. Flickinger
    • 1
  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of ColoradoBoulderUSA

Personalised recommendations