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Chromosoma

, Volume 103, Issue 1, pp 31–39 | Cite as

Sex chromosome polymorphism and heterogametic males revealed by two cloned DNA probes in the ZW/ZZ fish Leporinus elongatus

  • Ichiro Nakayama
  • Fausto Foresti
  • Rita Tewari
  • Manfred Schartl
  • Daniel Chourrout
Original Articles

Abstract

In order to study the divergence of teleost sex chromosomes, subtractive cloning was carried out between genomic DNA of males and females of the rainbow trout (XX/XY) and of Leporinus elongatus (ZW/ZZ). Inserts cloned in a plasmid vector were individually tested on Southern blots of DNA of males and females for sex specificity. No sex-specific insert was obtained from trout, but two out of ten inserts cloned from L. elongatus showed sex-specific patterns in this species: one corresponds to a sequence present on both Z and W chromosomes, while the other is W specific. Sequences of these two inserts show neither clear homology with other known sequences, nor an open reading frame. They cross-hybridize with the genomic DNA of Leporinus friderici, but without sex-specific patterns. Twenty-four L. elongatus adults were sexed by gonadal observation, chromosomed examination and Southern hybridization with one or the other insert. Ten males and 11 females had chromosomes and hybridization patterns typical of their sex. One ZW female was recognized as a male with the W-specific probe. This was also the case for two unusual ZW males, one having a male hybridization pattern with the other probe. These three atypical individuals may result from single genetic exchanges between four regions of the Z and the W, giving rise to three atypical W chromosomes. Finding males with such atypical heterochromosomes in a female heterogametic species may indicate that a gradual transition occurs between the heterogametic systems.

Keywords

Rainbow Trout Southern Blot Plasmid Vector Southern Hybridization Gradual Transition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Ichiro Nakayama
    • 1
  • Fausto Foresti
    • 2
  • Rita Tewari
    • 1
  • Manfred Schartl
    • 3
  • Daniel Chourrout
    • 1
  1. 1.Laboratoire de Génétique des PoissonsInstitut National de la Recherche AgronomiqueJouy en JosasFrance
  2. 2.Departamento de Morfologia, CEPUmversidade Estadual Paulista “Julio de Mesquita Filho”BotucatuBrazil
  3. 3.Lehrstuhl Physiologische Chemie ITheodor-Boveri-Institut für Biowissenschaften der Universität WürzburgWürzburgGermany

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