, Volume 27, Issue 6, pp 449–455

MHC class II sequences of an HLA-DR2 narcoleptic

  • C. B. Lock
  • A. K. L. So
  • K. I. Welsh
  • J. D. Parkes
  • J. Trowsdale
Original Articles


Narcolepsy has a 98% association with the DR2-Dw2/DQw1 haplotype. To establish if a disease-specific allele is present in narcolepsy, a cDNA library was made from a B-cell line from a DR2,4/DQw1,3 narcoleptic. Clones encoding the two expressed DR2β chains, along with DQw1 α and β chains, were isolated and completely sequenced. The coding regions of these four genes were similar to published nucleotide and protein sequences from corresponding healthy controls, with some minor exceptions. The 3′ untranslated region of one of the DR2β genes in the narcoleptic was extended by 42 bp. Complete sequences were not available for DQw1.2 α or β from healthy individuals, but first domain nucleotide sequences showed only a single nonproductive difference in DQα. Partial protein sequences of both DQ α and β from published data were identical. Although the effects of minor differences cannot be ruled out completely, it is concluded that there are probably no narcolepsy-specific DRβ or DQ α/β sequences, and that the alleles found in narcolepsy are representative of those found in the healthy population.


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • C. B. Lock
    • 1
  • A. K. L. So
    • 2
  • K. I. Welsh
    • 3
  • J. D. Parkes
    • 1
  • J. Trowsdale
    • 2
  1. 1.Department of Neurology, King's College Hospital School of MedicineDentistry, and Institute of PsychiatryDenmark Hill, LondonUK
  2. 2.Human Immunogenetics LaboratoryImperial Cancer Research FundLincoln's Inn Fields, LondonUK
  3. 3.Tissue Typing, Guy's HospitalLondonUK
  4. 4.Royal Postgraduate Medical SchoolHammersmith HospitalLondonUK

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