Immunogenetics

, Volume 28, Issue 5, pp 362–364

Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization

  • Helal A. Ansari
  • Robert Hediger
  • Rudolf Fries
  • Gerald Stranzinger
Article

Abstract

The first gene assignment to a horse chromosome is reported for equine leucocyte antigen (ELA), the major histocompatibility complex of the horse. A cloned DNA sequence derived from a class I gene of the porcine major histocompatibility complex was used as a probe for an in situ hybridization experiment. We present the regional localization of ELA, using this sequence, to equine chromosome 20g14-q22.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alexander, A. J., Bailey, E., and Woodward, J. G.: Analysis of the equine lymphocyte antigen system by Southern blot hybridization. Immunogenetics 25: 47–54, 1987Google Scholar
  2. Antczak, D. F., Barger, B., Bailey, E., Bernoco, D., Bull, R. W., Byrns, G. R., Guerin, G., Lazary, S., McClure, J., Mottoroni, V. D., Symons, R., Templeton, J., and Varewyck, H.: Joint report of the Third International Workshop on Lymphocyte Alloantigens of the Horse. Anim Genet 17: 363–373, 1986Google Scholar
  3. Bailey, E., Antczak, D. F., Bernoco, D., Bull, R. W., Fister, R., Guerin, G., Lazary, S., Mattews, S., McClure, J., Meyer, J., Mottironi, V. D., and Templeton, J.: Joint report of the Second Internation al Workshop on Lymphocyte Alloantigens of the Horse. Anim Blood Groups Biochem Genet 15: 123–132, 1984Google Scholar
  4. Bernoco, D., Antczak, D. F., Bailey, E., Bull, K., Bull, R. W., Byrns, G., Guerin, G., Lazary, S., McClure, J., Templeton, J., and Varewyck, H.: Joint report of the Fourth International Workshop on Lymphocyte Alloantigens of the Horse. Anim Genet 18: 81–94, 1987Google Scholar
  5. Bull, R. W.: Joint report of the First International Workshop on Lymphocyte Alloantigens of the Horse. Anim Blood Groups Biochem Genet 14: 119–137, 1983Google Scholar
  6. Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983Google Scholar
  7. Feinberg, A. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266–267, 1984Google Scholar
  8. Ford, C. E., Pollock, D. L., and Gustavsson, I. (eds.): Proceedings of the first international conference for the standardization of banded karyotypes of domestic animals. Hereditas 92: 145–162, 1980Google Scholar
  9. Fries, R., Hediger, R., and Stranzinger, G.: Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization. Anim Genet 17: 287–294, 1986Google Scholar
  10. Guerin, G., Bertaud, M., Chardon, P., Geffrotin, C., Vaiman, M., and Cohen, D.: Molecular genetic analysis of the major histocompatibility complex in an ELA typed horse family. Anim Genet 18: 323–336, 1987Google Scholar
  11. Halldorsdottir, S., and Davies, B.: Restriction fragment length polymorphism (RFLP) in Icelandic horses observed by using human MHC class II probes. Anim Genet 18: 29–30, 1987Google Scholar
  12. Hansen, K. M.: The relative length of Q-band/Giemsa stained horse chromosomes. Sixth Eur Colloq Cytogenet Domest Anim, pp. 165–171, 1984Google Scholar
  13. Lin, C. C., Draper, P. N., and De Braekeeler, M.: High-resolution chromosomal localization of the β-gene of the human β-globin gene complex by in situ hybridization. Cytogenet Cell Genet 39: 269–274, 1985Google Scholar
  14. Rabin, M., Fries, R., Singer, D., and Ruddle, F. H.: Assignment of the porcine major histocompatibility complex to chromosome 7 by in situ hybridization. Cytogenet Cell Genet 39: 206–209, 1985Google Scholar
  15. Singer, D. S., Camerini-Otero, R. D., Satz, M. L., Osborne, B., Sachs, D., and Rudikoff, S.: Characterization of a porcine genomic clone encoding a major histocompatibility complex antigen: expression in mouse L cells. Proc Natl Acad Sci USA 79: 1403–1407, 1982Google Scholar
  16. Vaiman, M., Chardon, P., and Cohen, D.: DNA polymorphism in the major histocompatibility complex of man and various farm animals. Anim Genet 17: 113–133, 1986Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Helal A. Ansari
    • 1
  • Robert Hediger
    • 1
  • Rudolf Fries
    • 1
  • Gerald Stranzinger
    • 1
  1. 1.Institute of Animal Sciences, Animal Breeding Group, ETH-ZentrumSwiss Federal Institute of TechnologyZurichSwitzerland

Personalised recommendations