Immunogenetics

, Volume 28, Issue 5, pp 328–333 | Cite as

The human VpreB gene is located on chromosome 22 near a cluster of \({\text{V}}_{\lambda _1 } \) gene segments

  • Steven R. Bauer
  • Kay Huebner
  • Marcia Budarf
  • Janet Finan
  • Jan Erikson
  • Beverly S. Emanuel
  • Peter C. Nowell
  • Carlo M. Croce
  • Fritz Melchers
Article

Abstract

The chromosomal location of the human VpreB gene was determined by Southern blotting analysis of restriction enzyme-digested DNAs from a panel of 17 mouse-human somatic cell hybrids. The pattern of hybridization of a VpreB-specific probe in conjunction with earlier analysis of several marker genes allowed the following conclusions: 1) VpreB is on human chromosome 22 within band 22q11.2 distal to the bcr-like gene, bcr-2 and proximal to the bcr-like gene, bcr-4. 2) VpreB has been localized relative to several constitutional and tumor-specific breakpoints within 22q 11.2, segregates in hybrids retaining 22qchromosomes with some but not with all members of the \({\text{V}}_{\lambda _1 } \) subgroup of the Vλgenes, and is amplified with these genes in K562 cells. 3) The order of the loci on chromosome 22 is centromere→bcr-2, VpreB, \({\text{V}}_{\lambda _1 } \to bcr - 4 \to C_\lambda \to bcr - 1 \to bcr - 3 \to sis\).

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Steven R. Bauer
    • 1
  • Kay Huebner
    • 2
  • Marcia Budarf
    • 3
  • Janet Finan
    • 4
  • Jan Erikson
    • 5
  • Beverly S. Emanuel
    • 3
  • Peter C. Nowell
    • 4
  • Carlo M. Croce
    • 2
  • Fritz Melchers
    • 1
  1. 1.Basel Institute for ImmunologyBaselSwitzerland
  2. 2.Wistar Institute of Anatomy and BiologyPhiladelphiaUSA
  3. 3.Department of PediatricsUniversity of Pennsylvania School of Medicine and Children's Hospital of PhiladelphiaPhiladelphiaUSA
  4. 4.Department of Pathology and Laboratory MedicineUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  5. 5.Institute for Cancer ResearchPhiladelphiaUSA

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