Mammalian Genome

, Volume 4, Issue 11, pp 627–631 | Cite as

Single-strand conformation polymorphism (SSCP) analysis as a tool for genetic mapping

  • David R. Beier
Review

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References

  1. Avner, P., Amar, L., Dandolo, L., Guénet, J.-L. (1988). Genetic analysis of the mouse using interspecific crosses. Trends Genet. 4, 18–23.Google Scholar
  2. Avramopoulos, D., Cox, T., Kraus, J.P., Charkravarti, A., Antonarakis, S.E. (1993). Linkage mapping of cystathione betasynthase (CBS) gene on human chromosome 21 using a DNA polymorphism in the 3′ untranslated region. Hum. Genet. 90, 566–568.Google Scholar
  3. Beier, D.R., Dushkin, H., Sussman, D.J. (1992). Mapping genes in the mouse using single strand conformation polymorphism analysis of recombinant inbred strains and interspecific crosses. Proc. Natl. Acad. Sci. USA 89, 9102–9106.Google Scholar
  4. Copeland, N.G., Jenkins, N.A. (1991). Development and applications of a molecular genetic linkage map of the mouse genome. Trends Genet. 7, 113–118.Google Scholar
  5. Dietrich, W., Katz, H., Lincoln, S.E., Shin, H.-S., Friedman, J., Dracopoli, N., Lander, E.S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–447.Google Scholar
  6. Dockhorn-Dworniczak, B., Dworniczak, B., Brommelkamp, L., Bulles, J., Horst, J., Bocker, W.W. (1991). Non-isotopic detection of single-strand conformation polymorphism (PCR-SSCP): a rapid and sensitive technique in the diagnosis of phenylketonuria. Nucleic Acids Res. 19, 2500.Google Scholar
  7. Goodwin, R.J., Anderson, D., Jerzy, R., Davis, T., Brannan, C.I., Copeland, N.G., Jenkins, N.A., Smith, C.A. (1991). Molecular cloning and expression of the Type 1 and Type 2 murine receptors for tumor necrosis factor. Mol. Cell. Biol. 11, 3020–3026.Google Scholar
  8. Hayashi, K. (1991). PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA. PCR Methods Aplic. 1, 34–38.Google Scholar
  9. Hayashi, K., Yandell, D.W. (1993). How sensitive is PCR-SSCP? Hum. Mutat., in press.Google Scholar
  10. Liang, P., Pardee, A.B. (1992). Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–970.Google Scholar
  11. Lisitsyn, N., Lisitsyn, N., Wigler, M. (1993). Cloning the differeneces between two complex genomes. Science 259, 946–951.Google Scholar
  12. Manley, K.F., Elliott, R.W. (1991). RI Manager, a microcomputer program for the analysis of data from recombinant inbred strains. Mammalian Genome 1, 123–126.Google Scholar
  13. Mohabeer, A., Hiti, A.L., Martin, W.J. (1991). Non-radioactive single strand conformation polymorphism (SSCP) using the Pharmacia “PhastSystem”. Nucleic Acids Res. 19, 3154.Google Scholar
  14. Nagamine, C.M., Chan, K., Lau, Y-F.C. (1989). A PCR artifact: generation of heteroduplexes. Am. J. Hum. Genet. 45, 337–339.Google Scholar
  15. Neilsen, D.A., Dean, M., Goldman, D. (1992). Genetic mapping of the human tryptophan hydroxylase gene on chromosome 11, using an intronic conformational polymorphism. Am. J. Hum. Genet. 51, 1366–1371.Google Scholar
  16. Nishimura, D.Y., Purchio, A.F., Murray, J.C. (1993). Linkage localization of TGFB2 and the human homeobox gene HLX1 to chromosome 1q. Genomics 15, 357–364.Google Scholar
  17. Orita, M., Suzuki, Y., Sekiya, T., Hayashi, K. (1989). Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5, 874–879Google Scholar
  18. Poduslo, S.E., Dean, M., Kolch, U., O'Brien, S.J. (1991). Detecting high-resolution polymorphisms in human coding loci by combining PCR and single-strand conformation polymorphism (SSCP) analysis. Am. J. Hum. Gen. 49, 106–111.Google Scholar
  19. Sheffield, V.C., Beck, J.S., Kwitek, A.E., Sandstrom, D.W., Stone, E.M. (1993). The sensitivity of single-strand conformation polymorphism analysis for the detection of single-base substitutions. Genomics 16, 325–332.Google Scholar
  20. Soto, D., Sukumar, S. (1992). Improved detection of mutations in the p53 gene in human tumors as single-stranded conformation polymorphs and double-stranded heteroduplex DNA. PCR Methods Applic. 2, 96–98.Google Scholar
  21. Spinardi, L., Mazars, R., Theillet, C. (1991). Protocols for an improved detection of point mutations by SSCP. Nucleic Acids Res. 19, 4009.Google Scholar
  22. Takahashi, N., Ko, M.S.H. (1993). The short 3′-end region of complementary DNAs as PCR-based polymorphic markers for an expression map of the mouse genome. Genomics 16, 161–168.Google Scholar
  23. Watson, M.L., D'Eustachio, P., Mock, B.A., Steinberg, A.D., H.C. Morse, I., Oakey, R.J., Howard, T.A., Rochelle, J.M., Seldin, M.F. (1992). A linkage map of mouse chromosome 1 using an interspecific cross segregating for the gld autoimmunity mutation. Mammalian Genome 2, 158–171.Google Scholar
  24. White, M.B., Carvalho, M., Derse, D., O'Brien, S.J., Dean, M. (1992). Detecting single-base substitutions as heteroduplex polymorphisms. Genomics 12, 301–306.Google Scholar

Copyright information

© Springer-Verlag New York Inc 1993

Authors and Affiliations

  • David R. Beier
    • 1
  1. 1.Genetics DivisionBrigham and Women's Hospital, Harvard Medical SchoolBostonUSA

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