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Chromosoma

, Volume 100, Issue 5, pp 330–338 | Cite as

Localization of specific repetitive DNA sequences in individual rice chromosomes

  • Hsin-Kan Wu
  • Mei-Chu Chung
  • Tiyun Wu
  • Chii-Nien Ning
  • Ray Wu
Article

Abstract

This paper describes the characterization and chromosomal distribution of three different rice (Oryza sativa) repetitive DNA sequences. The three sequences were characterized by sequence analysis, which gave 355, 498 and 756 bp for the length of the repeat unit in Os48, OsG3-498 and OsG5-756, respectively. Copy number determination by quantitative DNA slot-blot hybridization analysis showed 4000, 1080 and 920 copies, respectively, per haploid rice genome for the three sequences. In situ DNA hybridization analysis revealed that 95% of the silver grains detected with the Os48 probe were localized to euchromatic ends of seven long arms and one short arm out of the 12 rice chromosomes. For the OsG3-498 repetitive sequence, the majority of silver grains (58%) were also clustered at the same chromosomal ends as that of Os48. The minority (28%) of silver grains were located at heterochromatic short arms and centromeric regions. For the OsG5-756 repetitive sequence, 81% of the silver grains labeled the heterochromatic short arms and regions flanking all of the 12 centromeres. Thus, each of these three repetitive sequences was distributed at specific defined chromosomal locations rather than randomly at many chromosomal locations. The approximate copy number of a given repetitive DNA sequence at any specific chromosomal location was calculated by combining the information from in situ DNA hybridization analysis and the total copy number as determined by DNA slot-blot hybridization.

Keywords

Chromosomal Location Repetitive Sequence Centromeric Region Rice Chromosome Total Copy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Hsin-Kan Wu
    • 1
  • Mei-Chu Chung
    • 1
  • Tiyun Wu
    • 2
  • Chii-Nien Ning
    • 1
  • Ray Wu
    • 2
  1. 1.Institute of BotanyAcademia SinicaTaipeiTaiwan, ROC
  2. 2.Sect on of Biochemistry, Molecular and Cell BiologyCornell UniversityIthacaUSA

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