Chromosoma

, Volume 103, Issue 9, pp 642–652 | Cite as

A comparative study of karyotypes of muntjacs by chromosome painting

  • F. Yang
  • N. P. Carter
  • L. Shi
  • M. A. Ferguson-Smith
Article

Abstract

We have used a combination of chromosome sorting, degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), chromosome painting and digital image capturing and processing techniques for comparative chromosome analysis of members of the genus Muntiacus. Chromosome-specific “paints” from a female Indian muntjac were hybridised to the metaphase chromosomes of the Gongshan, Black, and Chinese muntjac by both single and three colour chromosome painting. Karyotypes and idiograms for the Indian, Gongshan, Black and Chinese muntjac were constructed, based on enhanced 4′, 6-diamidino-2-phenylindole (DAPI) banding patterns. The hybridisation signal for each paint was assigned to specific bands or chromosomes for all of the above muntjac species. The interspecific chromosomal homology was demonstrated by the use of both enhanced DAPI banding and comparative chromosome painting. These results provide direct molecular cytogenetic evidence for the tandem fusion theory of the chromosome evolution of muntjac species.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • F. Yang
    • 1
  • N. P. Carter
    • 2
  • L. Shi
    • 1
  • M. A. Ferguson-Smith
    • 2
  1. 1.Laboratory of Cellular and Molecular Evolution, Kunming Institute of ZoologyThe Chinese Academy of SciencesKunming, YunnanThe People's Republic of China
  2. 2.Department of PathologyUniversity of CambridgeCambridgeUK
  3. 3.The Sanger CentreCambridgeUK

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