Journal of Comparative Physiology B

, Volume 162, Issue 3, pp 197–202 | Cite as

Angiotensin II binding to tissues of the rainbow trout, Oncorhynchus mykiss, studied by autoradiography

  • Christopher S. Cobb
  • J. Anne Brown
Article
Accepted:

Summary

Tissue slices from seawater-adapted and freshwater-adapted rainbow trout, Oncorhynchus mykiss, were exposed to 125I-angiotensin II (1.01·10-9M) and binding sites located by light-microscopic autoradiography. Binding/uptake was significantly inhibited by excess (10-5M) unlabelled angiotensin II, suggesting specific binding/uptake of angiotensin II to the ventral and dorsal aorta (smooth muscle), urinary bladder (smooth muscle and epithelial lining), glomeruli and proximal tubules, the gill (lamellae and central filament), skin (epithelium), intestine and oesophagus (mucosal epithelium), liver, heart (ventricular myocytes), adrenocortical tissue and brain (cerebellum and medulla oblongata). The specific binding/uptake of angiotensin II to tissues of freshwater- and seawater-adapted animals were generally similar. However, binding/uptake by the proximal tubules was significantly higher in freshwater-adapted trout than seawater-adapted trout. Specific binding/uptake of angiotensin II by the smooth muscle of the bladder was significantly higher in trout adapted to seawater than trout adapted to freshwater.

Key words

Angiotensin II Autoradiography Seawater-adaptation trout, Oncorhynchus mykiss (= Salmogairdneri) 
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References

  1. Balment R, Carrick S (1985) Endogenous renin-angiotensin system and drinking behaviour in flounder. Am J Physiol 249:R157-R160Google Scholar
  2. Brown JA, Oliver JA, Henderson IW, Jackson BA (1980) Angiotensin and single nephron function in the trout Salmo gairdneri. Am J Physiol 239:R509-R514Google Scholar
  3. Brown JA, Taylor SM, Gray CJ (1990) Glomerular binding of angiotensin II in the rainbow trout, Salmo gairdneri. Cell Tissue Res 259:479–482Google Scholar
  4. Brown JA, Gray CJ, Hattersley G, Robinson J (1991) Prostaglandins in the kidnet, urinary bladder and gills of the rainbow trout and European eel adapted to fresh water and seawater. Gen Comp Endocrinol 84:328–335Google Scholar
  5. Decourt C, Lahlou B (1987) Evidence for the direct intervention of angiotensin II in the release of cortisol in teleost fishes. Life Sci 41:1517–1524Google Scholar
  6. Duncia JV, Chiu AT, Carini DJ, Gregory GB, Johnson AL, Price WA, Wells GJ, Wong PC, Calabrese JC, Timmermans PBMWM (1990) The discovery of potent nonpeptide angiotensin II receptor antagonists: a new class of potent antihypertensives. J Med Chem 33:1312–1329Google Scholar
  7. Fenwick JC, So YP (1981) Effect of an angiotensin on the net influx of calcium across the isolated perfused eel gill. Can J Zool 59:119–121Google Scholar
  8. Glossman H, Baukal A, Catt KJ (1974) Angiotensin II receptors in bovine adrenal cortex. Modification of angiotensin II binding by guanyl nucleotides. J Biol Chem 249:664–666Google Scholar
  9. Gray CJ, Brown JA (1985) Renal and cardiovascular effects of angiotensin II in the rainbow trout Salmo gairdner. Gen Comp Endocrinol 59:375–381Google Scholar
  10. Henderson IW, Hazon N, Hughes K (1985) Hormones, ionic regulation and kidney function in fishes. In: Laverack MS (ed) Physiological adaptation of marine animals. Symp Soc Exp Biol, vol XXXIX. Cambridge University Press, Cambridge, pp 245–262Google Scholar
  11. Hirano T (1980) Effects of cortisol and prolactin on ion permeability of the eel oesophagus. In: Lahlou B (ed) Epithelial transport in lower vertebrates. Cambridge University Press, Cambridge, pp 143–149Google Scholar
  12. Kobayashi H, Uemura H, Takei Y, Itatsu K, Ozawa M, Ichinohe K (1983) Drinking induced by angiotensin II in fishes. Gen Comp Endocrinol 44:295–306Google Scholar
  13. Lahlou B, Fossat B (1984) Transport properties of the fish urinary bladder in relation to osmoregulation. In: Pequeux A, Gilles R, Bolis L (eds) Osmoregulation in estuarine and marine organisms. Springer, New York Berlin Heidelberg, pp 93–106Google Scholar
  14. Levens NR, Peach MJ, Carey RM, Poat JA, Munday KA (1980) Stimulation of intestinal sodium and water transport in vitro by angiotensin II and analogues. Endocrinology 107:1946–1953Google Scholar
  15. Mendelsohn FAO (1985) Localisation and properties of angiotensin receptors. J Hypertens 3:307–316Google Scholar
  16. Nishimura H, Bailey JR (1982) Intrarenal renin-angiotensin system in primitive vertebrates. Kidney Int 22 [Suppl 12]:S185-S192Google Scholar
  17. Nishimura H, Norton VM, Bumpus FM (1978) Lack of specific inhibition of angiotensin II in eels by angiotensin antagonists. Am J Physiol 235:H95-H103Google Scholar
  18. Perrott MN, Balment RJ (1990) The renin-angiotensin system and the regulation of plasma cortisol in the flounder, Platichthys flesus. Gen Comp Endocrinol 78:414–420Google Scholar
  19. Rabkin R, Kitaji J (1983) Renal metabolism of peptide hormones. Miner Electrolyte Metab 9:212–226Google Scholar
  20. Skorecki KL, Ballermann BJ, Rennke HG, Brenner BM (1983) Angiotensin II receptor regulation in isolated renal glomeruli. Fed Proc 42:3064–3070Google Scholar
  21. Smith LS (1989) Digestive functions in teleost fishes. In: Halver JE (ed) Fish nutrition. Academic Press, New York, pp 332–423Google Scholar
  22. Takei Y, Hirano T, Kobayashi H (1979) Angiotensin and water uptake in the Japanese eel, Anguilla japonica. Gen Comp Endocrinol 38:466–475Google Scholar
  23. Takei Y, Okubo J, Yamaguchi K (1988) Effects of cellular dehydration on drinking and plasma angiotensin II level in the eel Anguilla japonica. Zool Sci 5:43–51Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Christopher S. Cobb
    • 1
  • J. Anne Brown
    • 1
  1. 1.Department of Biological Sciences, Hatherly LaboratoriesUniversity of ExeterExeterUK

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