Advertisement

Chromosoma

, Volume 101, Issue 2, pp 92–98 | Cite as

Antibodies directed against a meiosis-specific, chromatin-associated protein identify conserved meiotic epitopes

  • C. Daniel Riggs
  • Clare A. Hasenkampf
Original Articles

Abstract

The molecular mechanisms by which meiotic events are regulated are at present unknown. To approach this problem, we have exploited the natural synchrony of Lilium meiocytes to compare the nuclear protein profiles of a variety of stages of meiosis. This approach has facilitated the identification of a number of nuclear proteins that appear and disappear in a stagespecific fashion. Here we report the presence of an abundant nuclear protein that first appears during premeiotic interphase, a period during which the irreversible commitment to meiosis occurs. Antibodies directed against this protein demonstrate its meiosis specificity as well as conservation of the epitope(s) in both mono-and dicotyledonous plant species. Chromatin fractionation studies indicate that this protein, which we have termed meiotin-1, is associated with strings of nucleosomes. Implications for meiotic chromatin packaging and chromosome structure are discussed.

Keywords

Plant Species Molecular Mechanism Developmental Biology Nuclear Protein Protein Profile 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. AndersonLA, StackSM, FoxMH, ChuanshanZ (1985) The relationship between genome size and the synaptonemal complex length in higher plants. Exp Cell res 156:367–378Google Scholar
  2. AtchesonCL, DiDomenicoB, FrackmanS, EspositoRE, ElderRT (1987) Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene. Proc Natl Acad Sci USA 84:8035–8039Google Scholar
  3. ByersB, GoetschL (1975) Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae. Proc Natl Acad Sci USA 72:5056–5060Google Scholar
  4. CreightonHB, McClintockB (1931) A correlation of cytological and genetical crossing-over in Zea mays. Proc Natl Acad Sci USA 17:492–497Google Scholar
  5. EinarssonS, JosefssonB, LagerkvistS (1983) Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed phase high pressure liquid chromatography. J Chromatogr 282:609–618Google Scholar
  6. FawcettDW (1956) The fine structure of chromosomes in the meiotic prophase of vertebrate spermatocytes. J Biophys Biochem Cytol 2:403–406Google Scholar
  7. FinchJT, KlugA (1976) Solenoidal model for superstructure in chromatin. Proc Natl Acad Sci USA 73: 1897–1901Google Scholar
  8. GanttJS, KeyJL (1987) Molecular cloning of pea histone H1 cDNA. Eur J Biochem 166: 119–125Google Scholar
  9. GilliesCB (1984) The synaptonemal complex in higher plants. Crit Rev Plant Sci 2:81–116Google Scholar
  10. HasenkampfCA (1989) A method for producing synaptonemal complex complements in lily and mouse. J Heredity 80:197–202Google Scholar
  11. HollingsworthNM, ByersB (1989) HOP1: A yeast meiotic pairing gene. Genetics 121:445–462Google Scholar
  12. HollingsworthNM, GoetschL, ByersB (1990) The HOP1 gene encodes a meiosis-specific component of yeast chromosomes. Cell 61:73–84Google Scholar
  13. HuangS-Y, GarrardWT (1989) Electrophoretic analyses of nucleosomes and other protein-DNA complexes. Methods Enzymol 170:116–142Google Scholar
  14. ItoM, TakegamiMH (1982) Commitment of mitotic cells to meiosis during G2 phase of premeiosis. Plant Cell Physiol 23:943–952Google Scholar
  15. Jinks-RobertsonS, PetesTD (1986) Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics 114:731–752Google Scholar
  16. LaemmliUK, FavreM (1973) Maturation of the head of bacteriophage T4. J Mol Biol 80:575–599Google Scholar
  17. LowryOH, RosebroughNJ, FarrAL, RandallRJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  18. MatsudairaP (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262:10035–10038Google Scholar
  19. MoensPB, HeytingC, DietrichAJJ, VanRaamsdonkW, ChenQ (1987) Synaptonemal complex antigen location and conservation. J Cell Biol 105:93–103Google Scholar
  20. MosesMJ (1956) Chromosomal structures in crayfish spermatocytes. J Biophys Biochem Cytol 2:215–218Google Scholar
  21. NinnemannH, EpelB (1973) Inhibition of cell division by blue light. Exp Cell Res 79:318–326Google Scholar
  22. NollH, NollM (1989) Sucrose gradient techniques and applications to nucleosome structure. Methods Enzymol 170:55–115Google Scholar
  23. PanyinS, ChalkleyR (1971) The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method. J Biol Chem 246:7557–7560Google Scholar
  24. RattnerJB, GolsmithMR, HamkaloBA (1981) Chromosome organization during male meiosis in Bombyx mori. Chromosoma 82:341–351Google Scholar
  25. RockmillB, FogelS (1988) DIS 1: A yeast gene required for proper meiotic chromosome disjunction. Genetics 119:261–272Google Scholar
  26. RockmillB, RoederGS (1988) RED1: A yeast gene required for the segregation of chromosomes during the reductional division of meiosis. Proc Natl Acad Sci USA 85:6057–6061Google Scholar
  27. SheridanWF, SternH (1967) Histones of meiosis. Exp Cell Res 45:323–335Google Scholar
  28. SmithHE, MitchellAP (1989) A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae. Mol Cell Biol 9:2142–2152Google Scholar
  29. SternH (1990) Meiosis. In: AdolphKW (ed) Chromosomes: Eukaryotic, prokaryotic and viral, vol 2. CRC Press, Boca Raton, pp 3–37Google Scholar
  30. StrokovAA, BogdanovYF, ReznickovaSA (1993) A quantitative study of histones of meiocytes II. Polyacrylamide gel electrophoresis of isolated histones from Lilium microsporocytes. Chromosoma 43:247–260Google Scholar
  31. TousGI, FausnaughJL, AkinyosoyeO, LancklandH, Winter-CashP, VitoricaFJ, SteinS (1989) Amino acid analysis on polyvinylidene difluoride membranes. Anal Biochem 179:50–55Google Scholar
  32. vanHoldeKE (1989) Chromatin. Springer, Berlin Heidelberg New YorkGoogle Scholar
  33. vonWettsteinD, RasmussenSW, HolmPB (1984) The synaptonemal complex in genetic segregation. Annu Rev Genet 18:331–413Google Scholar
  34. WhitePR (1963) The cultivation of animal and plant cells, 2nd edn. Ronald Press, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • C. Daniel Riggs
    • 1
    • 2
  • Clare A. Hasenkampf
    • 1
  1. 1.Department of BotanyUniversity of Toronto, Scarborough CollegeScarboroughCanada
  2. 2.Centre for Plant BiotechnologyUniversity of Toronto, Scarborough CollegeScarboroughCanada

Personalised recommendations