Mammalian Genome

, Volume 5, Issue 6, pp 337–341 | Cite as

The mapping of transgenes by fluorescence in situ hybridization on G-banded mouse chromosomes

  • Y.-P. Shi
  • T.-T. Huang
  • E. J. Carlson
  • C. J. Epstein
Original Contributions
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Accepted:

Abstract

A highly sensitive method for the mapping of transgenes and other genes in the mouse genome is described. This technique combines high-resolution G-banding and fluorescence in situ hybridization (FISH) with either biotin/avidin-FITC or digoxigenin-anti-digoxigenin-FITC, the latter being the more sensitive. Banding patterns are obtained with trypsin/Geimsa-treated slides, and sensitivity is greatly increased by the use of mouse Cot-1 DNA. With this protocol, four different 14.5-kb human Cu/Zn-superoxide dismutase transgene insertions ranging in copy number from 2 to 8 have been localized to four different mouse chromosomes. The utility and sensitivity of this procedure were verified with a Chromosome (Chr) 16-specific cosmid probe, H22, as well as with the mapping of a high-copy-number human β-amyloid/A4 transgene.

Keywords

Banding Pattern Mouse Genome Mouse Chromosome Cosmid Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag New York Inc 1994

Authors and Affiliations

  • Y.-P. Shi
    • 1
  • T.-T. Huang
    • 1
  • E. J. Carlson
    • 1
  • C. J. Epstein
    • 1
  1. 1.Department of PediatricsUniversity of CaliforniaSan FranciscoUSA

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