Mammalian Genome

, Volume 6, Issue 8, pp 521–525 | Cite as

How bats achieve a small C-value: frequency of repetitive DNA in Macrotus

  • R. A. Van Den Bussche
  • J. L. Longmire
  • R. J. Baker
Original Contributions

Abstract

Bats possess a genome approximately 50–87% the size of other eutherian mammals. We document that the events that have achieved or maintained a small genome size in the Mexican leaf-nosed bat Macrotus waterhousii have resulted in a lower copy number of interspersed and tandemly repetitive elements. These conclusions are based on examination of 1726 randomly chosen recombinant cosmids, with an average insert size of 35.7 kb and representing 2.6% of the haploid genome of M. waterhousii. Probes representative of microsatellites [(GT)n, (CT)n, (AT)n, (GC)n] and a tandem repeat (rDNA) were used to estimate frequency of repetitive elements in the M. waterhousii genome. Of the four dinucleotides, (GT)n was present in 33.5% of the clones, (CT)n was present in 31.0% of the clones, and (AT)n and (GC)n were not represented in any of the clones examined. The 28S rDNA and a repetitive element from M. californicus were found in three and four clones, respectively. The dinucleotides (GT)n and (CT)n occurred together in the same clone more frequently than expected from chance. Although our data do not allow us to empirically test which mechanisms are maintaining copy number of repetitive DNA in the bat genome, the nonrandom association of these different families of repetitive DNA may provide insight into a mechanism that proportionately reduces diverse families of repetitive DNA that are known to be amplified by very different mechanisms.

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References

  1. Arnheim, N. (1979). Characterization of mouse ribosomal genome fragments purified by molecular cloning. Gene 7, 83–96.Google Scholar
  2. Bachmann, K. (1972). Genome size in mammals. Chromosoma 37, 85–93.Google Scholar
  3. Baker, R.J., Honeycutt, R.L., Van Den Bussche, R.A. (1991). Examination of monophyly of bats: restriction map of the ribosomal DNA cistron. In Contributions to Mammalogy in Honor of Karl F. Koopman, T.A. Griffiths, D. Klingener, eds. pp. 42–53. Bull. Am. Mus. Nat. Hist. 206, 1–432.Google Scholar
  4. Baker, R.J., Maltbie, M., Owen, J.G., Hamilton, M.J., Bradley, R.D. (1992). Reduced number of ribosomal sites in bats: evidence for a mechanism to contain genome size. J. Mammal. 73, 847–858.Google Scholar
  5. Baker, R.J., Longmire, J.L., Van Den Bussche, R.A. (1995). Organization of repetitive elements in the Upland cotton genome (Gossypium hirsutum). J. Hered. 86, 178–185.Google Scholar
  6. Beckman, J.S., Weber, J.L. (1992). Survey of human and rat microsatellites. Genomics 12, 627–631.Google Scholar
  7. Burton, D. W., Bickham, J. W., Genoways, H. H. (1989). Flow-cytometric analyses of nuclear DNA content in four families of Neotropical bats. Evolution 43, 756–765.Google Scholar
  8. Capanna, E., Manfredi-Romanini, M.G. (1971). Nuclear DNA content and morphology of the karyotype in certain Palearctic Microchiroptera. Caryologia 24, 471–481.Google Scholar
  9. Cavalier-Smith, T. (1985). Introduction: the evolutionary significance of genome size. In The Evolution of Genome Size, T. Cavalier-Smith, ed. (New York: John Wiley & Sons), pp. 1–36.Google Scholar
  10. Crampton, J.M., Davies, K.E., Knapp, T.P. (1981). The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes. Nucleic Acids Res. 9, 3821–3834.Google Scholar
  11. Evans, G.A., Lewis, K., Rothberg, B.E. (1989). High efficiency vectors for cosmid microcloning and genomic analysis. Gene 79, 9–20.Google Scholar
  12. Flavell, R.B., Bennett, M.D., Smith, J.B., Smith D.B. (1974). Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 12, 257–269.Google Scholar
  13. Hamada, H., Petrino, M.G., Kakunaga, T. (1982). A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proc. Natl. Acad. Sci. USA 79, 6465–6469.Google Scholar
  14. Hamilton, M.J., Honeycutt, R.L., Baker, R.J. (1990). Intragenomic movement, sequence amplification and concerted evolution in satellite DNA in harvest mice, Reithrodontomys: evidence from in situ hybridization. Chromosoma 99, 321–329.Google Scholar
  15. Hamilton, M.J., Hong, G., Wichman, H.A. (1992). Intragenomic movement and concerted evolution of satellite DNA in Peromyscus: evidence from in situ hybridization. Cytogenet. Cell Genet. 60, 40–44.Google Scholar
  16. Hinegardner, R. (1976). Evolution of genome size. In Molecular Evolution, F.J. Ayala, ed. (Sunderland, Mass.: Sinauer Associates, Inc.), pp. 179–199.Google Scholar
  17. Janecek, L.L., Longmire, J.L., Wichman, H.A., Baker, R.J. (1993). Organization of repetitive elements in the genome of the white-footed mouse, Peromyscus leucopus. Mamm. Genome 4, 374–381.Google Scholar
  18. Kato, H., Harada, M., Tsuchiya, K., Moriwaki, K. (1980). Absences of correlation between DNA repair in ultraviolet irradiated mammalian cells and lifespan of the donor species. Jpn. J. Genet. 55, 99–108.Google Scholar
  19. Lapitan, N.L.V. (1993). Organization and evolution of higher plant nuclear genomes. Genome 35, 171–181.Google Scholar
  20. Longmire, J.L., Ambrose, R.E., Brown, N.C., Cade, T.J., Maechtle, T.L., Seeger, W.S., Ward, F.P., White, C.M. (1991). Use of sex-linked minisatellite fragments to investigate genetic differentiation and migration of North American populations of the Peregrine Falcon (Falco peregrinus). In DNA Fingerprinting: Approaches and Applications, T. Burke, G. Dolf, A. Jeffreys, R. Wolff, eds. Brasil: Birkhauser Press), pp. 217–229.Google Scholar
  21. Longmire, J.L., Brown, N.C., Meoncke, Campbell, M.L., Albright, K.L., Fawcett, J.J., Campbell, E.W., Moyzis, R.K., Hildebrand, C.E., Evans, G.A., Deaven, L.L. (1993). Construction and characterization of partial digest libraries made from flow sorted human chromosome 16. Genet. Anal. Tech. Appl. 10, 69–76.Google Scholar
  22. Moyzis, R.K., Torney, D.C., Meyne, J., Buckingham, J.M., Wu, J.-R., Burk, C., Sirotkin, K.M., Goad, W.B. (1989). The distribution of interspersed repetitive DNA sequences in the human genome. Genomics 4, 273–289.Google Scholar
  23. Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Plainview, N.Y.: Cold Spring Harbor Laboratory Press).Google Scholar
  24. Shaeffer, R.L., Mendenhall, W., Ott, L. (1990). Elementary Survey Sampling, 4th ed. (Belmont, Calif.: Duxbury Press).Google Scholar
  25. Stallings, R.L. (1992). CpG suppression in vertebrate genomes does not account for the rarity of (CpG)n microsatellite repeats. Genomics 17, 1520–1521.Google Scholar
  26. Stallings, R.L., Ford, A.F., Nelson, D., Torney, D.C., Hildebrand, C.E., Moyzis, R. (1991). Evolution and distribution of (GT)n repetitive sequences in mammalian genomes. Genomics 10, 807–815.Google Scholar
  27. Swift, H. (1950). The constancy of deoxyribose nucleic acid in plant nuclei. Proc. Natl. Acad. Sci. USA 36, 643–654.Google Scholar
  28. Szarski, H. (1974). Cell size and nuclear DNA content in vertebrates. Int. Rev. Cytol. 23, 459–467.Google Scholar
  29. Tiersch, T.R., Wachtel, S.S. (1991). On the evolution of genome size in birds. J. Hered. 82, 363–368.Google Scholar
  30. Van Den Bussche, R.A. (1991). Phylogenetic analysis of restriction site variation in the ribosomal DNA complex of the New World leaf-nosed bat genera. Syst. Zool. 40, 420–432.Google Scholar
  31. Van Den Bussche, R.A. (1992). Restriction-site variation and molecular systematics of new World leaf-nosed bats. J. Mammal. 73:29–42.Google Scholar
  32. Wu, C-I., Hammer, M.F. (1991). Molecular evolution of ultraselfish genes of meiotic drive systems. In Evolution at the Molecular Level, R.K. Selander, A.G. Clark, T.S. Whittam, eds. (Sunderland, Mass.: Sinauer Associates, Inc.), pp. 177–203.Google Scholar

Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • R. A. Van Den Bussche
    • 1
  • J. L. Longmire
    • 1
  • R. J. Baker
    • 1
  1. 1.Department of Biological Sciences and The MuseumTexas Tech UniversityLubbockUSA

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