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Current Genetics

, Volume 11, Issue 4, pp 315–319 | Cite as

Curing of endogenous 2 micron DNA in yeast by recombinant vectors

  • M. N. Harford
  • M. Peeters
Article

Summary

Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with these plasmids offers a general method of obtaining ciro derivatives of any yeast strain.

Key words

2 micron DNA Curing S. cerevisiae Yeast vectors 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. N. Harford
    • 1
  • M. Peeters
    • 1
  1. 1.Molecular Genetics DepartmentSmith Kline - RITRixensartBelgium

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