Current Genetics

, Volume 11, Issue 4, pp 315–319 | Cite as

Curing of endogenous 2 micron DNA in yeast by recombinant vectors

  • M. N. Harford
  • M. Peeters


Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with these plasmids offers a general method of obtaining ciro derivatives of any yeast strain.

Key words

2 micron DNA Curing S. cerevisiae Yeast vectors 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Beggs JD (1978) Nature (London) 290:104–109Google Scholar
  2. Broach JR (1981) In: Strathern J, Jones EW, Broach JR (eds) Molecular biology of the yeast Saccharomyces, vol 2. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 445–470Google Scholar
  3. Broach JR, Hicks JB (1980) Cell 21:501–508Google Scholar
  4. Broach JR, Strathern JN, Hicks JB (1979) Gene 9:121–133Google Scholar
  5. Cashmore AM, Albury MS, Hadfield C, Meacock PA (1986) Mol Gen Genet 203:154–162Google Scholar
  6. Dobson MJ, Futcher AB, Cox BS (1980) Curr Genet 2:201–206Google Scholar
  7. Erhart E, Hollenberg CP (1981) Curr Genet 3:83–89Google Scholar
  8. Erhart E, Hollenberg CP (1983) J Bacteriol 156:625–635Google Scholar
  9. Harford N, Gathoye AM (1985) DNA 4:80Google Scholar
  10. Hinnen A, Hicks JB, Fink GF (1978) Proc Natl Acad Sci USA 75:1929–1933Google Scholar
  11. Hollenberg CP (1982) In: Hofschneider PH, Goebel W (eds) Current topics in microbiology and immunology, vol 96. Springer, Berlin Heidelberg New York, pp 119–144Google Scholar
  12. Ito H, Fukada Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168Google Scholar
  13. Jarayam M, Sutton A, Broach JR (1985) Mol Cell Biol 5:2466–2475Google Scholar
  14. Johnston SA, Hopper JE (1982) Proc Natl Acad Sci USA 79:6971–6975Google Scholar
  15. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
  16. Southern EM (1975) J Mol Biol 98:503–517Google Scholar
  17. Walmsley RM, Gardner DC, Oliver SG (1983) Mol Gen Genet 192:361–365Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. N. Harford
    • 1
  • M. Peeters
    • 1
  1. 1.Molecular Genetics DepartmentSmith Kline - RITRixensartBelgium

Personalised recommendations