Current Genetics

, Volume 11, Issue 4, pp 295–301 | Cite as

A mitochondrial frameshift-suppressor (+) of the yeast S. cerevisiae maps in the mitochondrial 15S rRNA locus

  • Brigitte Weiss-Brummer
  • Hajime Sakai
  • Fritz Kaudewitz
Article

Summary

The first case of a +1 “extrageneic” frameshift suppressor (MF1), mapping in the yeast mitochondrial 15S rRNA gene is reported. The suppressor was identified by genetic analyses in a leaky mitochondrial oxi1 frameshift mutant and the respective wild-type strain 777-3A of the yeast S. cerevisiae. This is in accordance with the finding that all mitochondrial frameshift mutants isolated from this strain tend to be leaky to a variable degree. MF1 does not suppress known nonsense mutations created by a direct basepair exchange in strain 777-3A. These mutants exhibit a non-leaky phenotype (Weiss-Brummer et al. 1984).

Key words

Yeast Mitochondrial Frameshift-Suppression 15S rRNA 

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References

  1. Atkins JF, Nicols BP, Thompson S (1983) EMBO J 2:1345–1350Google Scholar
  2. Avner PR, Coen D, Dujon B, Slonimski PP (1973) Mol Gen Genet 125:9–52Google Scholar
  3. Backhaus B, Schweyen RJ, Kaudewitz F (1978) Mol Gen Genet 161:153–173Google Scholar
  4. Burger G, Lang B, Bandlow W, Schweyen RJ, Backhaus B, Kaudewitz F (1976) Biochem Biophys Res Commun 72:1201–1207Google Scholar
  5. Claire J, Farabough P (1985) Proc Natl Acad Sci USA 82:2829–2833Google Scholar
  6. Coen D, Deutsch J, Netter P, Petrochilo E, Slonimski PP (1970) Control of organelle development. In: Miller PL (ed) Mitochondrial genetics. I. Methodology and phenomenology. University Press, Cambridge, pp 449–496Google Scholar
  7. Craigen WJ, Cook RG, Tate WP, Caskey CT (1985) Proc Natl Acad Sci USA 82:3616–3621Google Scholar
  8. Douglas M, Butow RA (1976) Proc Natl Acad Sci USA 73:1086–1093Google Scholar
  9. Dunn JJ, Studier FW (1983) J Mol Biol 166:477–535Google Scholar
  10. Fox TD (1979) J Mol Biol 130:63–82Google Scholar
  11. Fox TD, Staempfli S (1982) Proc Natl Acad Sci USA 79:1583–1587Google Scholar
  12. Fox TD, Weiss-Brummer B (1980) Nature (London) 288:60–63Google Scholar
  13. Gallant J, Foley D (1980) On the causes and prevention of mistranslation. In: Chambliss G, Craven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosome structure, function and genetics. University Press, Baltimore, pp 615–638Google Scholar
  14. Haid A, Schweyen RJ, Kaudewitz F, Solioz M, Schatz G (1979) Eur J Biochem 94:451–464Google Scholar
  15. Jacks T, Varmus HE (1985) Science 230:1237–1242Google Scholar
  16. Kotylak Z, Slonimski PP (1977) Fine structure genetic map of the mitochondrial DNA region controlling coenzyme QH2 — cytochrome c reductase. In: Bandlow W, Schweyen RJ, Wolf K, Kaudewitz F (eds) Genetics and biogenesis of mitochondria. de Gruyter, Berlin, pp 161–172Google Scholar
  17. Kováč L, Lachowicz TM, Slonimski PP (1967) Science 158:1564–1567Google Scholar
  18. Kruszewska A, Slonimski PP (1984) Curr Genet 9:11–19Google Scholar
  19. Kutzleb R, Schweyen RJ, Kaudewitz F (1973) Mol Gen Genet 125:91–98Google Scholar
  20. Lancashire WE, Mattoon JR (1979) Mol Gen Genet 170:333–344Google Scholar
  21. Li M, Tzagoloff A (1982) J Biol Chem 257:5921–5928Google Scholar
  22. Noller HF, Woese CR (1981) Science 212:403–411Google Scholar
  23. Ryoji M, Hsia K, Kaji A (1983) TIBS, pp 88–99Google Scholar
  24. Schweyen RJ, Weiss-Brummer B, Backhaus B, Kaudewitz F (1978) Mol Gen Genet 159:151–160Google Scholar
  25. Sibler A-P, Dirheimer G, Martin RP (1986) FEBS Lett 194:131–138Google Scholar
  26. Weiss RB (1984) Proc Natl Acad Sci USA 81:5797–5801Google Scholar
  27. Weiss-Brummer B, Guba R, Haid A, Schweyen RJ (1979) Curr Genet 1:75–83Google Scholar
  28. Weiss-Brummer B, Hüttenhofer A, Kaudewitz F (1984) Mol Gen Genet 198:62–68Google Scholar
  29. Wesolowski M, Fukuhara H (1979) Mol Gen Genet 170:261–275Google Scholar
  30. Wolf K, Dujon B, Slonimski PP (1973) Mol Gen Genet 125:53–90Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Brigitte Weiss-Brummer
    • 1
  • Hajime Sakai
    • 1
  • Fritz Kaudewitz
    • 1
  1. 1.Institut für Genetik und Mikrobiologie, Lehrstuhl für GenetikUniversität MünchenMünchen 19Germany

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