Molecular and General Genetics MGG

, Volume 121, Issue 1, pp 1–7 | Cite as

In vitro repression of N-α-acetyl-L-ornithinase synthesis in Escherichia coli

  • Elo Urm
  • Huey Yang
  • Geoffrey Zubay
  • Norman Kelker
  • Werner Maas
Article

Summary

Development of a system for in vitro synthesis of N-α-acetyl-L-ornithinase of E. coli has made it possible to detect the argR gene product, i.e., the arginine repressor, in cell extracts.

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References

  1. Celis, T.F.R., Maas, W.K.: Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. IV. Further studies on the role of arginine transfer RNA repression of the enzymes of arginine biosynthesis. J. molec. Biol. 62, 179–188 (1971).Google Scholar
  2. Elseviers, D., Cunin, R., Glansdorff, N., Baumberg, S., Ashcroft, E.: Control regions within the argECBH gene cluster of Escherichia coli K12. Molec. gen. Genet. 117, 349–366 (1972).Google Scholar
  3. Glansdorff, N.: Le contrôle génétique des biosynthèses de l'arginine et du carbamoylphosphate chez Escherichia coli. Thesis, University of Brussels (1966).Google Scholar
  4. Hirshfield, I.N., DeDeken, R., Horn, P.C., Hopwood, D.A., Maas, W.K.: Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. III. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants. J. molec. Biol. 35, 83–93 (1968).Google Scholar
  5. Jacoby, G.A.: Control of the argECBH cluster in Escherichia coli. Molec. gen. Genet. 117, 337–348 (1972).Google Scholar
  6. Jacoby, G.A., Gorini, L.: A unitary account of the repression mechanism of arginine biosynthesis in Escherichia coli. I. The genetic evidence. J. molec. Biol. 39, 73–87 (1969).Google Scholar
  7. Lowry, O., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).Google Scholar
  8. Press, R., Glansdorff, N., Miner, P., DeVries, J., Kadner, R., Maas, W.K.: Isolation of transducing particles of ϕ80 bacteriophage that carry different regions of the Escherichia coli genome. Proc. nat. Acad. Sci. (Wash.) 68, 795–798 (1971).Google Scholar
  9. Udaka, S.: Isolation of the arginine repressor in Escherichia coli. Nature (Lond.) 228, 336–338 (1970).Google Scholar
  10. Vogel, H.J., Bonner, D.M.: Acetyl ornithinase of Escherichia coli: Partial purification and some properties. J. biol. Chem. 218, 97–106 (1956).Google Scholar
  11. Vogel, R.H., McLellan, W.L., Hirvonen, A.P., Vogel, H.J.: The arginine biosynthesic system and its regulation. In: Metabolic regulation V. 463–488, ed. by H. Vogel. New York: Academic Press 1971.Google Scholar
  12. Zubay, G., Chambers, D., Cheong, L.: Cell-free studies in the regulation of the lac operon. In: The lactose operon, p. 375–391, ed. by J. Beckwith, D. Zipser, Cold Spring Harbor Laboratory, 1970.Google Scholar
  13. Zubay, G., Morse, D.E., Schrenk, W.J., Miller, J.H.M.: Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 69, 1100–1103 (1972).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Elo Urm
    • 1
  • Huey Yang
    • 1
  • Geoffrey Zubay
    • 1
  • Norman Kelker
    • 2
  • Werner Maas
    • 2
  1. 1.Department of Biological SciencesColumbia UniversityNew YorkUSA
  2. 2.Department of MicrobiologyNew York University, School of MedicineNew YorkUSA

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