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Current Genetics

, Volume 22, Issue 5, pp 345–355 | Cite as

Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase

  • B. Y. Tréton
  • M. -T. Le Dall
  • C. M. Gaillardin
Original Articles

Summary

A Yarrowia lipolytica gene library was constructed in vector YRp7 and transformed into a Saccharomyces cerevisiae strain lacking both major acid phosphatase activities. A 2.18 kb genomic sequence restoring the ability to hydrolyze α-naphthyl phosphate was isolated. Its sequencing revealed an ORF encoding 358 amino acids without significant homology with any known phosphatase. A putative signal peptide and several possible sites for N-glycosylation were identified. Phosphate-regulated expression of the cloned gene was observed in Y. lipolytica. Disruption data favoured the hypothesis that it might encode a minor phosphatase species.

Key words

Acid phosphatase Heterologous complementation Yarrowia lipolytica 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • B. Y. Tréton
    • 1
  • M. -T. Le Dall
    • 1
  • C. M. Gaillardin
    • 1
  1. 1.INRA, Laboratoire de Génétique Moléculaire et CellulaireCentre de Biotechnologies Agro-Industrielles, INA-PGThiverval-GrignonFrance

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