Chromosoma

, Volume 104, Issue 1, pp 39–43 | Cite as

Floral chromosomes of Arabidopsis thaliana for detecting low-copy DNA sequences by fluorescence in situ hybridization

  • M. Murata
  • F. Motoyoshi
Original Articles
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Abstract

Cosmid and plasmid clones containing 11 kb, or more, of genomic DNA sequences were mapped with high efficiencies using fluorescence in situ hybridization (FISH) to mitotic metaphase chromosomes prepared from floral tissues of Arabidopsis thaliana. The chromosomal locations were correlated with the map positions determined by RFLP (restriction fragment length polymorphism) analyses. Almost no signals were detected on the chromosomes of root meristematic tissues when FISH was performed with the same clones as probes. This discrepancy in efficiency of detection is possibly caused by the differences in chromatin structure between the root meristematic tissues and the floral tissues.

Keywords

Arabidopsis Thaliana Fragment Length Length Polymorphism Restriction Fragment Length Polymorphism Restriction Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • M. Murata
    • 1
  • F. Motoyoshi
    • 1
  1. 1.Research Institute for BioresourcesOkayama UniversityKurashikiJapan

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