Current Genetics

, Volume 23, Issue 2, pp 108–114 | Cite as

Mating configurations in Schizosaccharomyces pombe strains of different geographical origins

  • Thomas Schlake
  • Herbert Gutz
Original Articles
Received:

Abstract

In genetic research with Schizosaccharomyces pombe the strains used are almost exclusively descendants of the clones originally isolated by Leupold. In the “standard” homothallic (h90) strain three closely linked mating-type (MT) genes are present in the MT region: the actual MT locus, mat1, and two silent cassettes, mat2 and mat3, respectively. Various rearrangements are known in the MT region, e.g., heterothallic h+ or h- strains arise by duplications or deletions. In the present paper we analysed the mating behavior and the configurations of the MT regions of 19 S. pombe isolates from different parts of the world. In comparison with the Leupold strains several new MT configurations were found.

Key words

Mating-type genes Mating-type regions Schizosaccharomyces pombe 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beach DH (1983) Nature 305:683–688Google Scholar
  2. Beach DH, Klar AJS (1984) EMBO J 3:603–610Google Scholar
  3. Egel R (1989) In: Nasim A, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, San Diego, New York, pp 31–73Google Scholar
  4. Engelke U, Grabowski L, Gutz H, Schmidt H (1987) Curr Genet 12:535–542Google Scholar
  5. Fleck O, Heim L, Gutz H (1990) Curr Genet 18:501–509Google Scholar
  6. Gutz H, Doe FJ (1975) Mycologia 67:748–759Google Scholar
  7. Gutz H, Schmidt H (1985) Curr Genet 9:325–331Google Scholar
  8. Gutz H, Schmidt H (1990) Sem Develop Biol 1:169–176Google Scholar
  9. Gutz H, Heslot H, Leupold U, Loprieno N (1974) In: King, RC (ed) Handbook of genetics, vol 1. Plenum Press, New York, pp 395–446Google Scholar
  10. Heim L (1986) Dissertation, Naturwissenschaftliche Fakultät, Technische Universität, BraunschweigGoogle Scholar
  11. Heim L, Gutz H (1991) Curr Genet 19:435–444Google Scholar
  12. Kelly M, Burke J, Smith M, Klar AJS, Beach DH (1988) EMBO J 7:1537–1547Google Scholar
  13. Leupold U (1950) CR Trav Lab Carlsberg Sér Physiol 24:381–480Google Scholar
  14. Maniatis T, Jeffrey A, Kleid DG (1975) Proc Natl Acad Sci USA 72:1184–1188Google Scholar
  15. Schmidt H, Kapitza-Fecke P, Stephen ER, Gutz H (1989) Curr Genet 16:89–94Google Scholar
  16. Sipiczki M, Kucsera J, ulaszewski S, Zsolt J (1982) J Gen Microbiol 128:1989–2000Google Scholar
  17. Southern EM (1975) J Mol Biol 98:503–517Google Scholar
  18. Stephen ER, Nasim A (1981) Can J Microbiol 27:550–553Google Scholar
  19. Wright APH, Maundrell K, Shall S (1986) Curr Genet 10:503–508Google Scholar
  20. Zimmer M, Welser F, Oraler G, Wolf K (1987) Curr Genet 12:329–336Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Thomas Schlake
    • 1
  • Herbert Gutz
    • 1
  1. 1.Institut für GenetikTechnische Universität BraunschweigBraunschweigGermany

Personalised recommendations