Current Genetics

, Volume 25, Issue 5, pp 465–468 | Cite as

The ade4 gene of Schizosaccharomyces pombe: cloning, sequence and regulation

  • K. M. Ludin
  • N. Hilti
  • M. E. Schweingruber
Short Communications


We report the isolation and sequence of the Schizosaccharomyces pombe ade4 gene which encodes the glutamine phosphoribosylpyrophosphate amidotransferase, the first enzyme of the purine nucleotide de-novo biosynthetic pathway. The enzyme contains 533 amino acids and its sequence exhibits homologies to the corresponding enzymes of Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, chicken, rat, and human. In contrast to the situation in S. cerevisiae, adenine does not repress ade4 expression at the mRNA level and also other nutritional signals seem not to affect its expression.

Key words

PRPP amidotransferase ade4 gene Adenine Schizosaccharomyces pombe 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • K. M. Ludin
    • 1
  • N. Hilti
    • 1
  • M. E. Schweingruber
    • 1
  1. 1.Institute of General MicrobiologyUniversity of BerneBerneSwitzerland

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