Current Genetics

, Volume 25, Issue 5, pp 407–411

Isolation and characterization of three mutants with increased sensitivity to photoactivated 3-carbethoxypsoralen in Saccharomyces cerevisiae

  • C. B. Querol
  • S. O. Paesi-Toresan
  • L. B. Meira
  • M. Brendel
  • J. A. P. Henriques
Original Articles

Abstract

The complementation and genetical analysis of yeast mutants sensitive to photoactivated 3-carbethoxy-psoralen define three novel recessive mutant alleles pso-5-1, pso6-1, and pso7-1. Their cross-sensitivity to UV254nm, radiomimetic mutagens, and to chemicals enhancing oxidative stress suggest that these mutants are either impaired in metabolic steps protecting from oxidative stress or in mechanisms of the repair of oxygen-dependent DNA lesions. None of the three novel mutant alleles block the induction of reverse mutation by photoactivated mono- and bi-functional psoralens, nitrogen mustards, or UV254nm.

Key words

Psoralen sensitivity Saccharomyces cerevisiae DNA repair Oxidative stress 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • C. B. Querol
    • 1
  • S. O. Paesi-Toresan
    • 1
  • L. B. Meira
    • 1
  • M. Brendel
    • 2
  • J. A. P. Henriques
    • 1
  1. 1.Departamento de Biofisica, Centro de BiotecnologiaUFRGSPorto AlegreBrasil
  2. 2.Institut für MikrobiologieJ. W. Goethe-UniversitätFrankfurt/MainGermany
  3. 3.Departamento de Biologia Celular e GeneticaUniversidade Estadual de MaringaMaringaPR, Brasil

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