Current Genetics

, Volume 22, Issue 3, pp 229–234

Mitochondrial DNA sequence analysis of the cytochrome oxidase subunit I and II genes, the ATPase9 gene, the NADH dehydrogenase ND4L and ND5 gene complex, and the glutaminyl, methionyl and arginyl tRNA genes from Trichophyton rubrum

  • Claude de Bièvre
  • Bernard Dujon
Original Articles

Summary

In this paper, we present the nucleotide sequence of a 5248 bp-long region of the mitochondrial (mt) genome of the dermatophyte Trichophyton rubrum. This region which represents about 1/4 of the total mt genome of this species reveals a compact organization of genes including: the glutaminyl tRNA, the methionyl tRNA, the cytochrome oxidase subunit 1 gene, the arginyl tRNA, the mitochondrial version of the ATPase subunit 9 gene, the cytochrome oxidase subunit II gene and a part of the NADH dehydrogenase ND4L and ND5 gene “complex”. The main features of the part of mt DNA sequenced is the non-interrupted COXI gene and the presence in the mitochondrial version of the ATPase 9 gene of a small group IA intron. The extensive amino-acid sequence similarity with the equivalent gene in Aspergillus nidulans and neuropora crassa indicates that this gene codes for a dicyclohexylcarbodiimide binding protein. The conserved arrangement of this portion of the mt genome and the presence of tRNAs between the protein-coding genes are compatible with a large polycistronic transcript processed by the excision of tRNAs, or similar secondary structures, as proposed for other fungal or mammalian mt DNAS.

Key words

ATPase 9 Cytochrome oxidase DNA nucleotide sequence Intron Mitochondria NADH dehydrogenase tRNA Trichophyton rubrum 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Claude de Bièvre
    • 1
  • Bernard Dujon
    • 2
  1. 1.Unité de MycologieInstitut PasteurParis CedexFrance
  2. 2.Unité de Génétique Moléculaire des Levures (URA1149 du CNRS)Institut PasteurParis CedexFrance

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