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Current Genetics

, Volume 24, Issue 6, pp 465–471 | Cite as

Isolation of a mutant allele that deregulates the threonine biosynthesis in Saccharomyces cerevisiae

  • Encarna Martin-Rendon
  • Maria José Farfán
  • Cayo Ramos
  • Isabel L. Calderon
Original Articles

Abstract

We have cloned the yeast allele HOM3-R2, that codes for a mutant aspartate kinase which is insensitive to feedback inhibition by threonine, by gap-repair. A strain carrying this allele in a multicopy plasmid, or integrated into the genome, accumulates 14-times and 8-times more threonine than the wild-type, respectively. The sequence of the mutant allele differs from that of the wild-type in a single base pair change, namely a G by an A, at position 1355 in the open reading frame. The fact that the presence of this mutant allele in a cell induces threonine overproduction points to aspartate kinase as the key enzyme in the regulation of threonine biosynthesis in yeast.

Key words

S. cerevisiae Threonine biosynthesis Aspartate kinase Cloning of mutant allele 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Encarna Martin-Rendon
    • 1
  • Maria José Farfán
    • 1
  • Cayo Ramos
    • 2
  • Isabel L. Calderon
    • 1
  1. 1.Departamento de Genética, Facultad de BiologíaUniversidad de SevillaSevillaSpain
  2. 2.Carlsberg LaboratoryDepartment of Yeast GeneticsDK-CopenhagenDenmark

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