Current Genetics

, Volume 21, Issue 4–5, pp 345–349

Construction of a Tetracycline-inducible promoter in Schizosaccharomyces pombe

  • Khaschayar Faryar
  • Christiane Gatz
Original Articles

DOI: 10.1007/BF00351693

Cite this article as:
Faryar, K. & Gatz, C. Curr Genet (1992) 21: 345. doi:10.1007/BF00351693

Summary

We have developed a tightly repressed Schizosaccharomyces pombe promoter which can be efficiently induced by Tetracycline. This promoter is a derivative of the plant viral cauliflower mosaic virus 35S promoter which normally functions as a strong constitutive promoter in S. pombe. Location of three binding sites for the Tn10-encoded Tet repressor in the vicinity of the TATA-box of the CaMV 35S promoter led to a tight repression of promoter activity in the presence of the Tet repressor protein. Up to a 400-fold induction was observed after addition of the inducer Tetracycline, which inactivates the operator-binding capacity of the repressor.

Key words

CaMV 35S promoter Tet repressor Tetracycline induction Schizosaccharomyces pombe 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Khaschayar Faryar
    • 1
  • Christiane Gatz
    • 1
  1. 1.Institut für Genbiologische Forschung Berlin GmbHBerlin 33Federal Republic of Germany

Personalised recommendations